New DNA viruses identified in patients with acute viral infection syndrome
New DNA viruses identified in patients with acute viral infection syndrome
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DOI：
10.1128/jvi.79.13.8230-8236.2005
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发表时间：
2005-07-01
影响因子：
5.4
通讯作者：
Delwart, E
中科院分区：
文献类型：
--
作者：
Jones, MS;Kapoor, A;Delwart, E
使用序列非依赖性PCR扩增方法鉴定25名在人类免疫缺陷病毒1型传播高危行为后出现急性病毒感染症状的个体血浆样本中的病毒核酸。在3名个体中鉴定出GB病毒C/庚型肝炎病毒，在1名个体中鉴定出B型肝炎病毒。还检测到三种以前未描述的DNA病毒，一种细小病毒和两种与TT病毒（TTV）相关的病毒。在人类血浆中的核酸是远亲的细菌序列或没有检测到的相似性，已知sequences. Nearly完整的病毒基因组测序和系统发育分析证实了存在一种新的细小病毒不同已知的人类和动物细小病毒和两个相关的TTV-样病毒高度分歧的TTV和TTV样微小病毒组。在一小群病因不明的急性病毒综合征患者中检测到两种以前未描述的病毒种属，表明使用简单的非序列依赖性核酸扩增和有限测序方法可以容易地鉴定出大量新的人类病毒。
A sequence-independent PCR amplification method was used to identify viral nucleic acids in the plasma samples of 25 individuals presenting with symptoms of acute viral infection following high-risk behavior for human immunodeficiency virus type 1 transmission. GB virus C/hepatitis G virus was identified in three individuals and hepatitis B virus in one individual. Three previously undescribed DNA viruses were also detected, a parvovirus and two viruses related to TT virus (TTV). Nucleic acids in human plasma that were distantly related to bacterial sequences or with no detectable similarities to known sequences were also found. Nearly complete viral genome sequencing and phylogenetic analysis confirmed the presence of a new parvovirus distinct from known human and animal parvoviruses and of two related TTV-like viruses highly divergent from both the TTV and TTV-like minivirus groups. The detection of two previously undescribed viral species in a small group of individuals presenting acute viral syndrome with unknown etiology indicates that a rich yield of new human viruses may be readily identifiable using simple methods of sequence-independent nucleic acid amplification and limited sequencing.