METABOLISM OF PACHYTENE PRIMARY SPERMATOCYTES FROM RAT TESTES - PYRUVATE MAINTENANCE OF ADENOSINE-TRIPHOSPHATE LEVEL
METABOLISM OF PACHYTENE PRIMARY SPERMATOCYTES FROM RAT TESTES - PYRUVATE MAINTENANCE OF ADENOSINE-TRIPHOSPHATE LEVEL
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DOI：
10.1095/biolreprod30.5.1187
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发表时间：
1984-01-01
影响因子：
3.6
通讯作者：
ARAI, K
中科院分区：
文献类型：
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作者：
NAKAMURA, M;OKINAGA, S;ARAI, K
粗线期初级精母细胞的制备和检查能量代谢。当细胞与各种底物（葡萄糖，果糖，丙酮酸和乳酸）孵育，以测量其利用底物和ATP合成的程度，观察到精母细胞使用丙酮酸一样多的乳酸，但在数量上远远大于葡萄糖或果糖。丙酮酸和乳酸维持精母细胞内ATP水平，而只有乳酸维持精细胞周围和残留体内ATP水平。丙酮酸（5 mM）没有抑制ATP的合成，从精母细胞中的乳酸，不同的精子细胞中的行为。丙酮酸在克雷布斯循环中被氧化，用于进一步氧化磷酸化，并且不因添加葡萄糖而改变。丙酮酸和乳酸相互转化，但丙酮酸的细胞内水平保持不变（约0.2 mM）。精母细胞的乳酸脱氢酶（LDH）活性在0.3- 0.7mM丙酮酸时达到最大值，该酶促进丙酮酸向乳酸的转化，而不是相反的反应。糖酵解酶的活性测定和糖酵解中间产物的浓度测定表明，甘油醛-3-磷酸脱氢酶（GA 3 PDH）可能在糖酵解过程中起调节作用。精母细胞明显不同于精子细胞方面的能量代谢，即使在2类或germinal细胞的糖酵解大概是由GA 3 PDH调节。
Pachytene primary spermatocytes were prepared and examined for energy metabolism. When the cells were incubated with various substrates (glucose, fructose, pyruvate and lactate) to measure their utilization of substrates and the degree of ATP synthesis, spermatocytes were observed to use pyruvate as much as lactate, but in amounts much greater than those of glucose or fructose. Pyruvate and lactate maintained ATP levels in spermatocytes, while only lactate did in around spermatids and residual bodies. Pyruvate (5 mM) did not inhibit ATP synthesis from lactate in spermatocytes, differing from its behavior in spermatids. Pyruvate was oxidized in the Krebs cycle for further oxidative phosphorylation and was not altered by addition of glucose. Pyruvate and lactate were interconverted, but the intracellular level of pyruvate remained unchanged (.apprx. 0.2 mM) when eihter pyruvate or lactate was added to the medium. The maximal activity of lactate dehydrogenase (LDH) of spermatocytes occurred at 0.3-0.7 mM pyruvate, and this enzyme promoted the conversion of pyruvate to lactate rather than the reverse reaction. Activity assays of glycolytic enzyme and concentration assays of glycolytic intermediates showed a possible regulatory role for glyceraldehyde-3-phosphate dehydrogenase (GA3PDH) in glycolysis. Spermatocytes apparently differed strikingly from spermatids with respect to energy metabolism, even though glycolysis in the 2 categories or germinal cells was presumably regulated by GA3PDH.