DNA Metabolism and Cell Proliferation
DNA Metabolism and Cell Proliferation
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DNA 代谢和细胞增殖
DOI：
10.1007/978-1-4899-7321-4_8
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发表时间：
1969
期刊：
影响因子：
1.9
通讯作者：
J. Altman
中科院分区：
文献类型：
--
作者：
J. Altman
在考虑中枢神经系统的DNA新陈代谢和细胞增殖问题时，我们将在本章中回顾用三种主要技术获得的结果：(1)细胞分裂的组织学和细胞学研究，在某些研究中结合使用有丝分裂毒物(如秋水仙碱)阻断有丝分裂的辅助程序；(2)不同物种、不同发育阶段或不同脑区DNA浓度差异的大体和微观化学研究；以及(3)将放射性标记的特定DNA前体注射到动物身上以标记新形成的细胞的研究，这些细胞被呈现为可见的，并通过自动放射照相原位追踪它们的命运。因为在这一主题的历史上，组织学方法先于其他两种方法，我们将通过简要描述早期组织学研究的结果来介绍这一主题。这些早期研究产生的概念对这一领域的调查产生了持久的影响，但这些概念已得到补充、修改，并在某种程度上被用更现代技术获得的结果所取代。
In considering the problem of DNA metabolism and cell proliferation in the central nervous system we shall review in this chapter results obtained with three major techniques: (1) histological and cytological studies of cell division, which in some investigations were coupled with the auxiliary procedure of blocking mitosis with a mitotic poison, such as colchicine; (2) gross and microchemical studies of differences in DNA concentration in the brain in different species, at different phases of development or in different regions of the brain; and (3) studies in which a radioactively labeled specific precursor of DNA was administered to animals to tag newly forming cells and these were rendered visible and their fate traced in situ with auto-radiography. Because in the history of this topic the histological approach antedated the other two, we shall introduce the subject by describing briefly the results of early histological studies. These early studies generated concepts that had a lasting influence on investigations in this area, but these have been supplemented, modified and, to some extent, superseded by results obtained with more modern techniques.