SINGLET OXYGEN INDUCED DNA DAMAGE AND MUTAGENICITY IN A SINGLE-STRANDED SV40-BASED SHUTTLE VECTOR
SINGLET OXYGEN INDUCED DNA DAMAGE AND MUTAGENICITY IN A SINGLE-STRANDED SV40-BASED SHUTTLE VECTOR
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DOI：
10.1111/j.1751-1097.1992.tb04207.x
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发表时间：
1992-01-01
影响因子：
3.3
通讯作者：
MENCK, CFM
中科院分区：
文献类型：
--
作者：
RIBEIRO, DT;MADZAK, C;MENCK, CFM
分析了由水溶性NDPO 2（3，3 '-（1，4-萘亚甲基）二丙酸二钠的内过氧化物）热分解产生的单线态氧（1 O2）对单链穿梭载体的影响。1 O2在单链DNA的磷酸二酯骨架中诱导比双链DNA高得多的断裂水平。这可能是由于鸟嘌呤残基的可及性较高，主要由1 O2破坏。将受损的载体转染到猴COS 7细胞中，其中单链DNA转化为双链复制形式DNA。3天后，提取染色体外DNA并在E. coli进行诱变研究。与未处理的DNA相比，受损的单链DNA的突变频率显著增加。它的结论是，1 O2诱导断裂的单链DNA的骨干和1 O2损伤的分子突变后，通过哺乳动物细胞。
The effects of singlet oxygen (1O2), generated by the thermal decomposition of water soluble NDPO2 (endoperoxide of the disodium 3,3'-(1,4-naphthylidene) dipropionate), on a single-stranded shuttle vector were analysed. 1O2 induces a much higher level of breaks in the phosphodiester backbone of single-stranded than double-stranded DNA. This may be due to a higher accessibility of guanine residue, primarily damaged by 1O2. The damaged vector was transfected into monkey COS7 cells where single-stranded DNA was converted to the double-stranded replicative form DNA. After 3 days, extrachromosomal DNA was extracted and the plasmids rescued in E. coli to study mutagenesis. There is a significant increase in mutation frequency of damaged single-stranded DNA in comparison to untreated DNA. It is concluded that 1O2 induces breaks in the backbone of single-stranded DNA and that the 1O2-damaged molecules are mutated after passage through mammalian cells.