Production of spikeless particles of the rabies virus under conditions of low pH.
Production of spikeless particles of the rabies virus under conditions of low pH.
复制标题
在低 pH 条件下产生狂犬病病毒无刺颗粒。
DOI：
10.1016/0042-6822(81)90435-9
复制
发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
S. Matsumoto
中科院分区：
文献类型：
--
作者：
A. Kawai;S. Matsumoto
狂犬病病毒在低pH的培养基中生长导致产生大量无刺病毒粒子，其缺乏从病毒表面突出的糖蛋白。这些病毒体在pH低于7.0时首次出现，并在pH 6.7至6.8时达到最大值。无刺病毒粒子的沉降速度比感染性病毒粒子低，但在形状和大小上与感染性病毒粒子相似。我们目前的工作假设是，这些无刺病毒粒子是由蛋白水解消化后，在细胞膜上的标准病毒粒子的装配糖蛋白的大部分。这是基于以下发现：（1）感染细胞的电子显微照片显示，在大多数细胞相关病毒粒子上观察到刺突，其中三分之二的子代病毒粒子属于无刺突型;（2）无刺病毒粒子的SDS-PAGE显示一种新的低分子量多肽，并且在其中刺突已被链霉蛋白酶处理切割掉的感染性病毒体中也检测到显示出类似迁移率的多肽;和（3）两种低分子量多肽的肽图谱显示相似的结果。培养基的低pH值在产生无刺病毒中的意义正在研究中。
The growth of rabies virus at a low pH of the culture medium resulted in the production of a large number of spikeless virions which lacked glycoprotein protruding from the viral surface. These virions were first evident at a pH below 7.0 and reached a maximum at pH 6.7 to 6.8. The spikeless virion exhibits a lower sedimentation velocity than the infectious virion, but is similar to the infectious virion in shape and size. Our present working hypothesis is that these spikeless virions are formed by proteolytic digestion of a large portion of glycoprotein after the assembly of standard virions at the cellular membrane. This is based on the following findings: (1) Electron micrographs of infected cells in which two-thirds of the progeny virions belonged to the spikeless type revealed that spikes were observed on most cell-associated virions; (2) SDS-PAGE of the spikeless virions showed a new low molecular weight polypeptide, and a polypeptide showing a similar mobility was also detected in the infectious virions in which spikes had been cleaved off by Pronase treatment; and (3) peptide mappings of both low molecular weight polypeptides revealed similar findings. The significance of the low pH of the culture medium in producing spikeless virus is under investigation.