Invasive potential of bacterial isolates associated with subclinical bovine mastitis
Invasive potential of bacterial isolates associated with subclinical bovine mastitis
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DOI：
10.1016/j.rvsc.2006.02.002
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发表时间：
2006-12-01
影响因子：
2.4
通讯作者：
Ochoa-Zarzosa, Alejandra
中科院分区：
文献类型：
--
作者：
Anaya-Lopez, Jose L.;Contreras-Guzman, Oscar E.;Ochoa-Zarzosa, Alejandra
这项工作描述了与乳房炎相关的细菌分离株侵袭能力的差异。侵袭能力取决于原代培养的牛乳腺上皮细胞(BMEC)的摄取和存活情况。从一头健康哺乳奶牛中分离出BMEC，并对其进行了形态、细胞角蛋白免疫染色以及β-和kappa-酪蛋白mRNAs的检测。从后院养殖场奶牛乳汁中分离到金黄色葡萄球菌(3株)、表皮葡萄球菌(1株)、溶血葡萄球菌(1株)、马氏葡萄球菌(2株)、木质葡萄球菌(1株)和站短杆菌(2株)等10株细菌，对其侵袭BMEC的能力进行了检测。只有两株金黄色葡萄球菌和一株表皮葡萄球菌能够侵袭骨髓内皮细胞，其水平与金黄色葡萄球菌对照菌株ATCC 27543相似。总之，使用本研究中使用的体外感染模型，可以检测到细菌侵袭能力的差异。(C)2006爱思唯尔有限公司。保留所有权利。
This work describes differences in the invasive ability of bacterial isolates associated with mastitis. Invasion ability was determined by the uptake and survival in a primary culture of bovine mammary epithelial cells (BMEC). BMEC were isolated from a healthy lactating cow and characterized by their morphology, immunostaining for cytokeratin and the detection of beta- and kappa-casein mRNAs. Ten bacterial isolates comprising the staphylococcal species Staphylococcus aureus (3), Staphylococcus epidermidis (1), Staphylococcus haemolyticus (1), Staphylococcus equorum (2), Staphylococcus xylosus (1) and Brevibacterium stationis (2) obtained from raw milk of cows with mastitis from backyard farms were assayed for their ability to invade BMEC. Only two S. aureus and one S. epidermidis isolates were able to invade BMEC, at similar levels to the S. aureus control strain ATCC 27543. In conclusion, using the in vitro model of infection used in this study, differences in bacterial invasion capability may be detected. (c) 2006 Elsevier Ltd. All rights reserved.