Formulation and characterization of poloxamine-based hydrogels as tissue sealants.
Formulation and characterization of poloxamine-based hydrogels as tissue sealants.
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DOI：
10.1016/j.actbio.2012.03.003
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发表时间：
2012-07
影响因子：
9.7
通讯作者：
Webb K
中科院分区：
文献类型：
--
作者：
Cho E;Lee JS;Webb K
原位可交联聚乙二醇 (PEG) 基聚合物作为密封剂在外科手术中发挥着越来越重要的作用，可防止液体/气体泄漏和粘连形成。本研究研究了由两种丙烯酸酯化泊洛沙胺（Tetronics® T1107 和 T904）的均质和混合溶液形成的水凝胶的凝胶行为和物理性质，相对于 PEG 对照，这两种丙烯酸酯化泊洛沙胺具有不同的分子量和亲水/亲油平衡。水凝胶是通过在生理温度下反向热凝胶化（含有T1107的制剂）并通过与二硫苏糖醇的迈克尔型加成进行共价交联而形成的。所有基于泊洛沙明的水凝胶都表现出热敏行为，并在生理温度下相对于 PEG 水凝胶实现了显着减少的膨胀、增加的拉伸性能和增加的组织粘合强度。所有基于泊洛沙明的水凝胶在 37°C 时的溶胀和拉伸性能均明显高于 4°C，这表明它们的物理性能改善源自非共价和共价机制的协同交联。基于泊洛沙明的水凝胶具有细胞相容性，并且根据其 T1107/T904 成分，会在 2 至 5 周内发生水解降解。总之，精选的泊洛沙明基水凝胶具有许多可能有益于组织密封剂应用的特性，包括室温/生理温度之间粘度的大幅增加、对细胞粘附的抵抗力以及在平衡过程中保持稳定的体积。
In-situ crosslinkable polyethylene glycol (PEG)-based polymers play an increasing role in surgical practice as sealants that provide a barrier to fluid/gas leakage and adhesion formation. This study investigated the gelation behavior and physical properties of hydrogels formed from homogeneous and blended solutions of two acrylated poloxamines (Tetronics® T1107 and T904) of varying molecular weight and hydrophilic/lipophilic balance relative to a PEG control. Hydrogels were formed by reverse thermal gelation at physiological temperature (T1107-containing formulations) and covalent crosslinking by Michael-type addition with dithiothreitol. All poloxamine-based hydrogels exhibited thermosensitive behavior and achieved significantly reduced swelling, increased tensile properties, and increased tissue bond strength relative to the PEG hydrogel at physiological temperature. Swelling and tensile properties of all poloxamine-based hydrogels were significantly greater at 37 °C relative to 4 °C, suggesting that their improved physical properties derive from cooperative crosslinking by both noncovalent and covalent mechanisms. Poloxamine-based hydrogels were cytocompatible and underwent hydrolytic degradation over 2 to 5 weeks depending on their T1107/T904 composition. In conclusion, select poloxamine-based hydrogels possess a number of properties potentially beneficial to tissue sealant applications including substantial increase in viscosity between room/physiological temperatures, resistance to cell adhesion, and maintenance of stable volume during equilibration.