Structure of the capsular polysaccharide of Acinetobacter baumannii ACICU containing di-N-acetylpseudaminic acid
Structure of the capsular polysaccharide of Acinetobacter baumannii ACICU containing di-N-acetylpseudaminic acid
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DOI：
10.1016/j.carres.2014.04.002
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发表时间：
2014-06-04
影响因子：
3.1
通讯作者：
Knirel, Yuriy A.
中科院分区：
文献类型：
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作者：
Senchenkova, Sof'ya N.;Shashkov, Alexander S.;Knirel, Yuriy A.
采用苯酚-水萃取法从鲍曼不动杆菌ACICU细胞中分离出荚膜多糖，通过糖分析、部分酸水解、1D和2D H-1和C-13核磁共振波谱对荚膜多糖进行研究。发现该多糖含有5,7-二乙酰氨基-3,5,7,9-四氧基- l -甘油- l -甘露-非2-醛酸或二n-乙酰伪胺酸（Pse5Ac7Ac），并建立了支链四糖重复单元的如下结构：[图]鲍曼a.p aumannii ACICU多糖基因簇中存在的基因与所建立的结构相适应。(C) 2014 Elsevier Ltd.版权所有。
Capsular polysaccharide was isolated by the phenol-water extraction of Acinetobacter baumannii ACICU cells and studied by sugar analysis, partial acid hydrolysis, and 1D and 2D H-1 and C-13 NMR spectroscopy. The polysaccharide was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic or di-N-acetylpseudaminic acid (Pse5Ac7Ac), and the following structure of the branched tetrasaccharide repeating unit was established:[GRAPHICS]The genes present in the polysaccharide gene cluster of A. baumannii ACICU are appropriate to the structure established. (C) 2014 Elsevier Ltd. All rights reserved.