Insect immunity. Isolation and sequence of two cDNA clones corresponding to acidic and basic attacins from Hyalophora cecropia
Insect immunity. Isolation and sequence of two cDNA clones corresponding to acidic and basic attacins from Hyalophora cecropia
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昆虫免疫力。
DOI：
10.1002/j.1460-2075.1984.tb02093.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
H. G. Boman
中科院分区：
文献类型：
--
作者：
K. Kockum;I. Faye;P. Hofsten;J;K. Xanthopoulos;H. G. Boman
天蚕蛾具有三种已知的抗菌免疫蛋白，即附着素、溶菌酶和天蚕素（先前分别称为P5、P7和P9）。纯化了六种不同等电点的亲和素。其中五种形式的N末端序列意味着只有两种不同的基因存在。我们现在已经分离并测序了两个cDNA克隆，一个是碱性attacin，一个是酸性attacin。两个成熟蛋白在核苷酸水平上显示76%的同源性，而终止密码子以外的区域是36%的同源性。天冬氨酸含量的差异解释了酸性和碱性attacin之间净电荷的差异。电荷的进一步差异可以通过翻译后去除两种蛋白质C末端的含赖氨酸四肽来获得。提出了基本attacin的一种预形式的证据。
The Cecropia moth has three known classes of antibacterial immune proteins, attacins, lysozyme and cecropins (earlier referred to as P5, P7 and P9, respectively). Six attacins with different isoelectric points have been purified. The N‐terminal sequences for five of these forms imply that only two different genes exist. We have now isolated and sequenced two cDNA clones, one for the basic attacin and one for the acidic form. The two mature proteins show 76% homology at the nucleotide level, while the regions beyond the stop codons are 36% homologous. The differences in the content of aspartic acid accounts for the difference in net charge between the acidic and basic attacin. Further differences in charge can be obtained by post‐translational removal of a lysine‐containing tetrapeptide at the C‐terminal end of the two proteins. Evidence for a prepro form of the basic attacin is presented.