SULFONYLUREA-RESISTANT MUTANTS OF ARABIDOPSIS-THALIANA
SULFONYLUREA-RESISTANT MUTANTS OF ARABIDOPSIS-THALIANA
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DOI：
10.1007/bf00331020
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
SOMERVILLE, C
中科院分区：
文献类型：
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作者：
HAUGHN, GW;SOMERVILLE, C
通过筛选除草剂对拟南芥幼苗生长的影响，获得了拟南芥的绿磺隆抗性突变体。整个植物和衍生组织培养物对这种除草剂的浓度都有抵抗力，大约是防止野生型生长所需浓度的300倍。这种抗性是由于一个名为CSR的基因座上的一个显性核突变造成的，该基因已被遗传定位在第三染色体上。与野生型相比，绿黄隆抗性植物提取物中的乙羟基酸合酶活性对绿磺隆和一种结构相关的抑制剂的抑制作用要小得多。这表明CSR基因是乙酰羟基酸合成酶的结构基因。
Chlorsulfuron-resistant mutants of Arabidopsis thaliana were isolated by screening for growth of seedlings in the presence of the herbicide. Both whole plants and derived tissue cultures were resistant to concentrations of the herbicide approximately 300-fold higher than that required to prevent growth of the wild-type. The resistance is due to a single dominant nuclear mutation at a locus designated csr which has been genetically mapped to chromosome-3. Acetohydroxy acid synthase activity in extracts from chlorsulfuron-resistant plants was much less-susceptible to inhibition by chlorsulfuron and a structurally related inhibitor than the activity in wild-type extracts. This suggests that the csr locus is the structural gene for acetohydroxy acid synthase.