Co-administration of Bacillus subtilis RJGP16 and Lactobacillus salivarius B1 strongly enhances the intestinal mucosal immunity of piglets
Co-administration of Bacillus subtilis RJGP16 and Lactobacillus salivarius B1 strongly enhances the intestinal mucosal immunity of piglets
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DOI：
10.1016/j.rvsc.2012.07.025
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发表时间：
2013-02-01
影响因子：
2.4
通讯作者：
Yang, Qian
中科院分区：
文献类型：
--
作者：
Deng, Jun;Li, Yunfeng;Yang, Qian
益生菌，包括芽孢杆菌。和乳酸菌，是猪饲用低剂量抗生素的潜在替代品。本研究旨在评价枯草芽孢杆菌RJGP16和唾液乳杆菌B1作为潜在益生菌联合应用刺激局部免疫应答的效果。将32头初生仔猪随机分为4组，分别在0、7、11日龄分别饲喂不同组合的益生菌(Non、RJGP16、B1、RJGP16和B1)。对断奶后一周仔猪的黏膜免疫指标进行分析。结果表明，RJGP16和B1合用后，十二指肠和回肠组织中IL-6和十二指肠组织中猪β-防御素(PBD)-2的基因表达显著增加(P<0.01)。TLR-2的表达和释放及免疫球蛋白(Ig)A产生细胞数增加(P<0.01)。结果表明，两种细菌联合给药比单独给药更能激发粘膜免疫力。(C)2012爱思唯尔有限公司。保留所有权利。
Probiotics, including Bacillus spp. and Lactobacillus, are potential replacements for low dose in-feed antibiotics for pig. This study aimed to evaluate the effect of the co-administration of Bacillus subtilis RJGP16 and Lactobacillus salivarius B1 as potential probiotics to stimulate local immune responses. Thirty two newborn piglets were divided into four groups and orally administrated with different combination of probiotics (none; RJGP16; B1; RJGP16 and B1) at the age of 0, 7 and 11 days. We analysed the parameters of the mucosal immunity of piglets a week after weaning. Our results showed that the gene expression of interleukin (IL)-6 in the duodenum and ileum, porcine beta-defensins (pBD)-2 in the duodenum were significantly increased (p < 0.01) with co-administration of the RJGP16 and B1. Also the expression and release of TLR-2 and the number of immunoglobulin (Ig) A producing cells were increased (p < 0.01). The results demonstrate that the co-administration of the two bacteria stimulate a more intense mucosal immunity than the administration of each bacterium alone. (C) 2012 Elsevier Ltd. All rights reserved.