Focusing and alignment of erythrocytes in a viscoelastic medium.
Focusing and alignment of erythrocytes in a viscoelastic medium.
复制标题
粘弹性介质中红细胞的聚焦和对齐。
DOI：
10.1038/srep41162
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发表时间：
2017-01-24
影响因子：
4.6
通讯作者：
Lee SJ
中科院分区：
文献类型：
--
作者：
Go T;Byeon H;Lee SJ
粘弹性流体流动引起的跨流线迁移最近受到了相当大的关注，因为这个过程提供了简单的聚焦和对齐在很宽的流速范围。颗粒的横向迁移取决于通道的几何形状和颗粒的物理化学性质。在这项研究中，数字同轴全息显微镜（DIHM）的研究人类红细胞的横向迁移的粘弹性流体流动诱导的矩形微通道。DIHM提供微通道中颗粒的3D空间分布和颗粒取向信息。在粘弹性流体的压力驱动流动中产生的弹性力将悬浮颗粒推离壁，并迫使红细胞具有固定的取向。血细胞的变形性影响微通道中的侧向聚焦和固定取向。与刚性球体和硬化红细胞不同，可变形的正常红细胞随着流速的增加从通道中心平面分散。此外，在通道侧壁附近的区域中，正常红细胞具有比硬化红细胞更高的倾斜角。这些结果对红细胞变形性异常引起的血液病的无标记诊断具有指导意义。
Viscoelastic fluid flow-induced cross-streamline migration has recently received considerable attention because this process provides simple focusing and alignment over a wide range of flow rates. The lateral migration of particles depends on the channel geometry and physicochemical properties of particles. In this study, digital in-line holographic microscopy (DIHM) is employed to investigate the lateral migration of human erythrocytes induced by viscoelastic fluid flow in a rectangular microchannel. DIHM provides 3D spatial distributions of particles and information on particle orientation in the microchannel. The elastic forces generated in the pressure-driven flows of a viscoelastic fluid push suspended particles away from the walls and enforce erythrocytes to have a fixed orientation. Blood cell deformability influences the lateral focusing and fixed orientation in the microchannel. Different from rigid spheres and hardened erythrocytes, deformable normal erythrocytes disperse from the channel center plane, as the flow rate increases. Furthermore, normal erythrocytes have a higher angle of inclination than hardened erythrocytes in the region near the side-walls of the channel. These results may guide the label-free diagnosis of hematological diseases caused by abnormal erythrocyte deformability.