Arsukibacterium indicum sp. nov., isolated from deep-sea sediment, and transfer of Rheinheimera tuosuensis and Rheinheimera perlucida to the genus Arsukibacterium as Arsukibacterium tuosuense comb. nov. and Arsukibacterium perlucidum comb. nov.
Arsukibacterium indicum sp. nov., isolated from deep-sea sediment, and transfer of Rheinheimera tuosuensis and Rheinheimera perlucida to the genus Arsukibacterium as Arsukibacterium tuosuense comb. nov. and Arsukibacterium perlucidum comb. nov.
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DOI：
10.1099/ijsem.0.005455
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发表时间：
2022-07
影响因子：
2.8
通讯作者：
Zhen Wang;Jun-Hui Cheng;Xiao-yu Zhang;Fei Xu;Hui-hui Fu;Chunyang Li;Yu‐Zhong Zhang;Xiulan Chen;Xiao-yan Song;Xi-Ying Zhang
中科院分区：
文献类型：
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作者：
Zhen Wang;Jun-Hui Cheng;Xiao-yu Zhang;Fei Xu;Hui-hui Fu;Chunyang Li;Yu‐Zhong Zhang;Xiulan Chen;Xiao-yan Song;Xi-Ying Zhang
从西南印度洋深海沉积物样品中分离到一株革兰氏阴性需氧鞭毛杆状细菌，命名为SM2107T。菌株SM2107T在4-40℃、0-10.0%(w/v)的氯化钠环境中生长。它将硝酸盐还原为亚硝酸盐，并水解酪蛋白、明胶、甲壳素和DNA。基于16S rRNA基因和单拷贝同源聚类构建的系统发育树表明，菌株SM2107T与陀螺莱姆氏菌(Rheinheimera Tuosuensis)、周氏莱茵海默氏菌(Rheinheimera PerLucida)和伊克森氏隐杆杆菌(Arsukitis Ikkense)形成了一个独立的分支，与拓苏氏模式菌(Rheinheimera Tuosuensis)的相似性最高(98.3%)。主要极性脂类为磷脂酰乙醇胺和磷脂酰甘油，主要细胞脂肪酸为特征8(C18：1ω7c和/或C18：1ω6c)、C16：0、C17：1ω8с和总和特征3(C16：1ω7c和/或C16：1ω6c)。唯一的呼吸性对苯二酚是Q-8。SM2107T菌株基因组DNA G+C含量为48.8%。SM2107T株与拓氏大黄菌、紫红大黄菌和伊氏古杆菌模式株的DNA-DNA数字杂交值分别为41.16、37.70和31.80%，它们之间的氨基酸平均同源性分别为87.59、86.76和83.64%。根据本研究中提出的多相证据，菌株SM2107T被认为代表了松材线虫属中的一个新种，并建议将其命名为印度松材线虫。模式菌株为SM2107T(=MCCC M24986T=KCTC 82921T)。此外，还将陀螺大黄菌和周氏大黄菌转移到松材线虫属，作为松材线虫Arsukibactuosuense comb.11月(模式菌株TS-T4T=CGMCC 1.12461T=JCM 19264T)；11月模式菌株BA131T=1MG23581T=CIP109200T。
A Gram-stain-negative, aerobic, flagellated and rod-shaped bacterium, designated strain SM2107T, was isolated from a deep-sea sediment sample collected from the Southwest Indian Ocean. Strain SM2107T grew at 4-40 °C and with 0-10.0 % (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed casein, gelatin, chitin and DNA. The phylogenetic trees based on the 16S rRNA genes and single-copy orthologous clusters showed that strain SM2107T, together with Rheinheimera tuosuensis, Rheinheimera perlucida and Arsukibacterium ikkense, formed a separate clade, having the highest similarity to the type strain of Rheinheimera tuosuensis (98.3%). The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol and the major cellular fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), C16 : 0, C17 : 1 ω8с and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c). The only respiratory quinone was Q-8. The genomic DNA G+C content of strain SM2107T was 48.8 %. The digital DNA-DNA hybridization values between strain SM2107T and type strains of Rheinheimera tuosuensis, Rheinheimera perlucida and Arsukibacterium ikkense were 41.16, 37.70 and 31.80 %, while the average amino acid identity values between them were 87.59, 86.76 and 83.64 %, respectively. Based on the polyphasic evidence presented in this study, strain SM2107T was considered to represent a novel species within the genus Arsukibacterium, for which the name Arsukibacterium indicum was proposed. The type strain is SM2107T (=MCCC M24986T=KCTC 82921T). Moreover, the transfer of Rheinheimera tuosuensis and Rheinheimera perlucida to the genus Arsukibacterium as Arsukibacterium tuosuense comb. nov. (type strain TS-T4T=CGMCC 1.12461T=JCM 19264T) and Arsukibacterium perlucidum comb. nov. (type strain BA131T=LMG 23581T=CIP 109200T) is also proposed.