Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.
Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.
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DOI：
10.14814/phy2.13893
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发表时间：
2018-10
影响因子：
2.5
通讯作者：
Fujita S
中科院分区：
文献类型：
--
作者：
Yokokawa T;Kido K;Suga T;Isaka T;Hayashi T;Fujita S
线粒体翻译的过程，其中线粒体（mt）DNA编码的基因被翻译成蛋白质，对线粒体功能和生物发生至关重要。在每个阶段，一系列线粒体翻译因子是合成mtDNA编码的线粒体蛋白所必需的。两个线粒体起始因子（mtIF 2和mtIF 3）、三个线粒体延伸因子（mtEFTu、mtEFT和mtEFG 1）、一个线粒体释放因子（mtRF 1 L）和两个线粒体再循环因子（mtRRF 1和mtRRF 2）是协调每个翻译阶段的线粒体翻译因子。运动增加核DNA和mtDNA编码的线粒体蛋白，导致骨骼肌中的线粒体生物合成。因此，线粒体翻译因子可能受运动调节;然而，尚不清楚运动是否影响骨骼肌中的线粒体翻译因子。我们研究了运动训练是否会全面增加骨骼肌中这一系列线粒体翻译因子以及mtDNA编码的蛋白质。将小鼠随机分配到久坐组或运动组，并在有或没有转轮的标准笼中饲养1周和8周。然后测量跖肌和比目鱼肌中线粒体翻译因子的表达水平。运动训练同时上调线粒体翻译因子和线粒体蛋白在跖肌。然而，在比目鱼肌，这些全面上调没有检测到。这些结果表明，运动诱导的线粒体生物发生与线粒体翻译因子的上调一致。
The process of mitochondrial translation, in which mitochondrial (mt)DNA‐encoded genes are translated into proteins, is crucial for mitochondrial function and biogenesis. In each phase, a series of mitochondrial translation factors is required for the synthesis of mtDNA‐encoded mitochondrial proteins. Two mitochondrial initiation factors (mtIF2 and mtIF3), three mitochondrial elongation factors (mtEFTu, mtEFTs, and mtEFG1), one mitochondrial release factor (mtRF1L), and two mitochondrial recycling factors (mtRRF1 and mtRRF2) are mitochondrial translation factors that coordinate each translational phase. Exercise increases both nuclear DNA‐ and mtDNA‐encoded mitochondrial proteins, resulting in mitochondrial biogenesis in skeletal muscles. Therefore, mitochondrial translation factors are likely regulated by exercise; however, it is unclear whether exercise affects mitochondrial translation factors in the skeletal muscles. We investigated whether exercise training comprehensively increases this series of mitochondrial translation factors, as well as mtDNA‐encoded proteins, in the skeletal muscle. Mice were randomly assigned to either the sedentary or exercise group and housed in standard cages with or without a running wheel for 1 and 8 weeks. The expression levels of mitochondrial translation factors in the plantaris and soleus muscles were then measured. Exercise training concomitantly upregulated mitochondrial translation factors and mitochondrial proteins in the plantaris muscle. However, in the soleus muscle, these comprehensive upregulations were not detected. These results indicate that exercise‐induced mitochondrial biogenesis coincides with the upregulation of mitochondrial translation factors.