Multiple-strain colonization in nasal carriers of Staphylococcus aureus.
Multiple-strain colonization in nasal carriers of Staphylococcus aureus.
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DOI：
10.1128/jcm.03254-13
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发表时间：
2014-04
影响因子：
9.4
通讯作者：
Walker AS
中科院分区：
文献类型：
--
作者：
Votintseva AA;Miller RR;Fung R;Knox K;Godwin H;Peto TE;Crook DW;Bowden R;Walker AS
金黄色葡萄球菌是一种常见的细菌，也可以引起侵袭性感染。报告表明鼻腔共定植很少发生，但需要对多个克隆进行测序所需的资源排除了大规模调查的可能性。采用英国牛津郡健康人群的纵向研究中的3,197个金黄色葡萄球菌阳性样本，开发了一种阶段性方案，以最大限度地检测到混合spa类型的定植，同时最大限度地减少实验室资源。对每个样本的混合材料进行初始分型，在89.6%的样本中发现了单一明确的菌株。从初步结果不明确的样本中对12株单菌落分离株进行了分型。使用该协议，可以将所有样品分解成一种或多种水疗类型。在360名招募阳性的患者中，在24个月的跟踪调查中，殖民地点流行率为3.4%至5.8%。然而，18%的人至少被同居一次，大多数只是短暂的。在56%的样本中，共殖化温泉类型完全无关。在2 72例重新采集的≥阳性标本中，16 6例(61%)持续携带金黄色葡萄球菌，但只有10 6例(39%)携带相同的单一spa型而没有任何共定植，31例(11%)转换spa型，29例(11%)有一过性共带。即使在长期携带者中，美国金黄色葡萄球菌的定植也是动态的。新的不相关的共生菌株可能会增加侵袭性疾病的风险，正在进行的宿主内进化可能会增加侵袭性潜力，这是未来研究应该探索的可能性。
Staphylococcus aureus is a commensal that can also cause invasive infection. Reports suggest that nasal cocolonization occurs rarely, but the resources required to sequence multiple colonies have precluded its large-scale investigation. A staged protocol was developed to maximize detection of mixed-spa-type colonization while minimizing laboratory resources using 3,197 S. aureus-positive samples from a longitudinal study of healthy individuals in Oxfordshire, United Kingdom. Initial typing of pooled material from each sample identified a single unambiguous strain in 89.6% of samples. Twelve single-colony isolates were typed from samples producing ambiguous initial results. All samples could be resolved into one or more spa types using the protocol. Cocolonization point prevalence was 3.4 to 5.8% over 24 months of follow-up in 360 recruitment-positives. However, 18% were cocolonized at least once, most only transiently. Cocolonizing spa types were completely unrelated in 56% of samples. Of 272 recruitment-positives returning ≥12 swabs, 166 (61%) carried S. aureus continuously but only 106 (39%) carried the same single spa type without any cocolonization; 31 (11%) switched spa type and 29 (11%) had transient cocarriage. S. aureus colonization is dynamic even in long-term carriers. New unrelated cocolonizing strains could increase invasive disease risk, and ongoing within-host evolution could increase invasive potential, possibilities that future studies should explore.