Differential accumulation of gangliosides by the brains of MPTP-lesioned mice.
Differential accumulation of gangliosides by the brains of MPTP-lesioned mice.
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MPTP 损伤小鼠大脑中神经节苷脂的差异积累。
DOI：
10.1002/jnr.490370310
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发表时间：
1994
影响因子：
4.2
通讯作者：
Schengrund,CL
中科院分区：
文献类型：
--
作者：
Saulino,MF;Schengrund,CL
尽管有报道称神经节苷脂可促进1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）诱导的黑质病变的恢复，但缺乏证据证明给予的神经节苷脂是否真的到达了中枢神经系统（CNS）中的该病变部位或任何其他病变部位，从而促进了恢复。因此，进行研究以确定MPTP处理小鼠的大脑以及对照小鼠的大脑中积累的3 H标记的神经节苷脂的量。在注射标记脂质后240 min内，在损伤和对照脑之间未观察到3/-牛脑神经节苷脂或3 H-GD 1a的蓄积存在显著差异。然而，在注射3 H-GM 1后120和240分钟，与对照组相比，MPTP治疗组小鼠的大脑中有更多的标记。对从3 H-GM 1处理的小鼠脑中提取的脂质进行分析，发现在给药后240分钟，大部分标记物仍与3-GM 1相关。注射3 H-GM 1的MPTP处理小鼠脑组织切片的放射自显影显示，标记物存在于脑室空间中。这一观察结果表明，给予的神经节苷脂存在于脑脊液中，这表明它们有可能到达受损的CNS部位，在该处它们可促进恢复。© 1994 Wiley利斯公司
Although gangliosides have been reported to enhance recovery from 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐induced lesions of the substantia nigra, evidence as to whether the administered gangliosides actually reach this or any other site of lesion in the central nervous system (CNS) at which they putatively enhance recovery is lacking. Therefore, studies were carried out to determine the amount of3H‐labeled ganglioside that was accumulated by the brains of MPTP‐treated mice as well as by brains of control mice. No significant difference in the accumulation of3/‐bovine brain gangliosides or3H‐GD1a was seen between lesioned and control brains up to 240 min after injection of the labeled lipids. However, significantly more label was associated with the brains of MPTP‐treated mice compared to controls 120 and 240 min after the injection of3H‐GM1. Analysis of the lipids extracted from the brain of a3H‐GM1‐treated mouse revealed that the majority of label was still associated with3‐GM1, 240 min after its administration. Autoradiography of tissue sections from the brains of MPTP‐treated mice injected with3H‐GM1 showed that label was present in the ventricular spaces of the brain. This observation suggests that the administered gangliosides are present in the cerebrospinal fluid, which indicates that they have the potential to reach the lesioned CNS site at which they putatively enhance recovery. © 1994 Wiley‐Liss, Inc.