Colistin and Polymyxin B Susceptibility Testing for Carbapenem-Resistant and mcr-Positive Enterobacteriaceae: Comparison of Sensititre, MicroScan, Vitek 2, and Etest with Broth Microdilution
Colistin and Polymyxin B Susceptibility Testing for Carbapenem-Resistant and mcr-Positive Enterobacteriaceae: Comparison of Sensititre, MicroScan, Vitek 2, and Etest with Broth Microdilution
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DOI：
10.1128/jcm.00268-17
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发表时间：
2017-09-01
影响因子：
9.4
通讯作者：
Teo, Jeanette W. P.
中科院分区：
文献类型：
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作者：
Chew, Ka Lip;La, My-Van;Teo, Jeanette W. P.
粘菌素和多粘菌素B仍然是多重耐药革兰氏阴性菌（如耐碳青霉烯类肠杆菌科）的最后一批抗生素。目前EUCAST-CLSI联合建议进行肉汤微量稀释（BMD），用于粘菌素的MIC检测。评价了市售药敏试验方法，并与参考BMD进行了比较，Sensititre（粘菌素斯皮尔曼的rho = 0.863，多粘菌素B斯皮尔曼的rho = 0.877）和Vitek 2（多粘菌素B [仅]斯皮尔曼的rho = 0.8917）达到了90%的药敏折点。尽管Vitek 2的粘菌素检测与参考方法的MIC呈正相关（斯皮尔曼rho = 0.873），但分类一致性为2 mg/L，其中17种的多粘菌素MIC通过肉汤微量稀释法>2 mg/L。使用较低的断点
Colistin and polymyxin B remain part of the last line of antibiotics for multidrug-resistant Gram-negative bacteria, such as carbapenem-resistant Enterobacteriaceae. Current joint EUCAST-CLSI recommendations are for broth microdilution (BMD) to be performed for MIC testing of colistin. Commercial susceptibility testing methods were evaluated and compared against the reference BMD, using a susceptibility breakpoint of 90% was achieved for Sensititre (colistin Spearman's rho = 0.863, and polymyxin B Spearman's rho = 0.877) and Vitek 2 (polymyxin B [only] Spearman's rho = 0.8917). Although a positive MIC correlation (Spearman's rho = 0.873) with the reference method was achieved for colistin testing with Vitek 2, categorical agreement was 2 mg/liter, and polymyxin MICs for 17 of them were >2 mg/liter by broth microdilution. The use of a lower breakpoint of