Nonlinear Strain Stiffening Is Not Sufficient to Explain How Far Cells Can Feel on Fibrous Protein Gels
Nonlinear Strain Stiffening Is Not Sufficient to Explain How Far Cells Can Feel on Fibrous Protein Gels
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DOI：
10.1016/j.bpj.2013.05.032
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发表时间：
2013-07-02
影响因子：
3.4
通讯作者：
Billiar, Kristen L.
中科院分区：
文献类型：
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作者：
Rudnicki, Mathilda S.;Cirka, Heather A.;Billiar, Kristen L.
最近的观察表明，纤维细胞外基质材料上的细胞比线性弹性合成材料上的细胞在更大的距离上感知机械信号。在这项工作中，我们系统地调查的距离成纤维细胞可以通过纤维凝胶感知刚性边界，通过量化的人肺成纤维细胞和3T3成纤维细胞培养在倾斜的胶原蛋白和纤维蛋白凝胶的扩散面积。当凝胶厚度从0增加到150 μ m时，细胞面积逐渐减小，在纤维蛋白和胶原蛋白凝胶下面具有>65 μ m的特征传感距离，并且在厚达150 μ m的凝胶上受到扩散影响。这些结果表明，成纤维细胞对胶原蛋白和纤维蛋白凝胶的感知比对聚丙烯酰胺凝胶的感知更深，后者表现出特征性的感知距离，
Recent observations suggest that cells on fibrous extracellular matrix materials sense mechanical signals over much larger distances than they do on linearly elastic synthetic materials. In this work, we systematically investigate the distance fibroblasts can sense a rigid boundary through fibrous gels by quantifying the spread areas of human lung fibroblasts and 3T3 fibroblasts cultured on sloped collagen and fibrin gels. The cell areas gradually decrease as gel thickness increases from 0 to 150 mu m, with characteristic sensing distances of >65 mu m below fibrin and collagen gels, and spreading affected on gels as thick as 150 mu m. These results demonstrate that fibroblasts sense deeper into collagen and fibrin gels than they do into polyacrylamide gels, with the latter exhibiting characteristic sensing distances of