MyCompoundID: Using an Evidence-Based Metabolome Library for Metabolite Identification
MyCompoundID: Using an Evidence-Based Metabolome Library for Metabolite Identification
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DOI：
10.1021/ac400099b
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发表时间：
2013-03-19
影响因子：
7.4
通讯作者：
Lin, Guohui
中科院分区：
文献类型：
--
作者：
Li, Liang;Li, Ronghong;Lin, Guohui
Identification of unknown metabolites is a major challenge in metabolomics. Without the identities of the metabolites, the metabolome data generated from a biological sample cannot be readily linked with the proteornic and genomic information for studies in systems biology and medicine. We have developed a web-based metabolite identification tool (http://www.mycompoundid.org) that allows searching and interpreting mass spectrometry (MS) data against a newly constructed metabolome library composed of 8 021 known human endogenous metabolites and their predicted metabolic products (375 809 compounds from one metabolic reaction and 10 583 901 from two reactions). As an example, in the analysis of a simple extract of human urine or plasma and the whole human urine by liquid chromatography-mass spectrometry and MS/MS, we are able to identify at least two times more metabolites in these samples than by using a standard human metabolome library. In addition, it is shown that the evidence-based metabolome library (EML) provides a much superior performance in identifying putative metabolites from a human urine sample, compared to the use of the ChemPub and KEGG libraries.
