Kinetics of amino sugar formation from organic residues of different quality
Kinetics of amino sugar formation from organic residues of different quality
复制标题
不同质量有机残渣形成氨基糖的动力学
DOI：
10.1016/j.soilbio.2012.08.006
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发表时间：
2013-02-01
影响因子：
9.7
通讯作者：
Boeckx, Pascal
中科院分区：
文献类型：
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作者：
Bai, Zhen;Bode, Samuel;Boeckx, Pascal
氨基糖是微生物细胞壁的关键化合物，已被广泛用作微生物残留物的生物标志物，以研究土壤微生物群落和有机残留物循环过程。然而，氨基糖的形成动力学尚不清楚。在本研究中，采用不同质量（籽粒、叶和根）的统一 C-13 标记的小麦残留物对两种不同耕作管理下的农业 Luvisol 进行了修正。使用液相色谱同位素比质谱 (LC-IRMS) 和痕量气体 IRMS 在 21 天的培养期内测量各个氨基糖的同位素组成和二氧化碳排放量。结果表明，残基衍生的氨基糖的量呈指数增加，并在添加残基后几天内达到最大值。氨基葡萄糖和半乳糖胺遵循不同的形成动力学。残基衍生的氨基糖形成的最大值范围为半乳糖胺的14 nmol g(-1)干土（原始浓度的0.8%）到氨基葡萄糖的319 nmol g(-1)干土（原始浓度的11%）。葡萄糖胺和半乳糖胺的残基衍生氨基糖的平均生产时间分别为 2.1 至 9.3 天。一般来说，随着植物残渣质量的提高，以更高的速率形成更大量的氨基糖。免耕土壤的微生物群落更适合吸收低质量的植物残留物（即叶子和根）。总而言之，微生物细胞壁成分的形成动力学具有成分特异性，并由残留物质量和土壤微生物群落决定。 (C) 2012 Elsevier Ltd. 保留所有权利。
Amino sugars are key compounds of microbial cell walls, which have been widely used as biomarker of microbial residues to investigate soil microbial communities and organic residue cycling processes. However, the formation dynamics of amino sugar is not well understood. In this study, two agricultural Luvisols under distinct tillage managements were amended with uniformly C-13-labeled wheat residues of different quality (grain, leaf and root). The isotopic composition of individual amino sugars and CO2 emission were measured over a 21-day incubation period using liquid chromatography isotope ratio mass spectrometry (LC-IRMS) and trace gas IRMS. Results showed that, the amount of residue derived amino sugars increased exponentially and reached a maximum within days after residue addition. Glucosamine and galactosamine followed different formation kinetics. The maxima of residue derived amino sugars formation ranged from 14 nmol g(-1) dry soil for galactosamine (0.8% of the original concentration) to 319 nmol g(-1) dry soil for glucosamine (11% of the original concentration). Mean production times of residue derived amino sugars ranged from 2.1 to 9.3 days for glucosamine and galactosamine, respectively. In general, larger amounts of amino sugars were formed at a higher rate with increasing plant residue quality. The microbial community of the no-till soil was better adapted to assimilate low quality plant residues (i.e. leaf and root). All together, the formation dynamics of microbial cell wall components was component-specific and determined by residue quality and soil microbial community. (C) 2012 Elsevier Ltd. All rights reserved.