Selective release, removal, and reconstitution of bacteriochlorophyll a molecules into the B800 sites of LH2 complexes from Rhodopseudomonas acidophila 10050
Selective release, removal, and reconstitution of bacteriochlorophyll a molecules into the B800 sites of LH2 complexes from Rhodopseudomonas acidophila 10050
复制标题
DOI：
10.1021/bi990796i
复制
发表时间：
1999-07-27
期刊：
影响因子：
2.9
通讯作者：
Cogdell, RJ
中科院分区：
文献类型：
--
作者：
Fraser, NJ;Dominy, PJ;Cogdell, RJ
描述了一种允许选择性地从嗜酸红假单胞菌10050的LH2复合体中释放和去除Bch1a-B800分子的方法。这一过程还允许与天然Bch1a重组类似80%的空结合位点。圆二色谱分析表明，B850单体和重组络合物的整体结构不受颜料交换过程的影响。重组到B800位的色素也能有效地将激发能传递给Bchla-B850分子。
A method is described which allows the selective release and removal of the Bchla-B800 molecules from the LH2 complex of Rhodopseudomonas acidophila 10050. This procedure also allows reconstitution of similar to 80% of the empty binding sites with native Bchla. As shown by circular dichroism spectroscopy, the overall structures of the B850-only and reconstituted complexes are not affected by the pigment-exchange procedure. The pigments reconstituted into the B800 sites can also efficiently transfer excitation energy to the Bchla-B850 molecules.