Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing.
Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing.
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DOI：
10.1038/srep27507
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发表时间：
2016-06-06
影响因子：
4.6
通讯作者：
Gu X
中科院分区：
文献类型：
--
作者：
Hao Y;Cui Y;Gu X
热应激影响食用动物的肌肉发育和肉质;然而，关于其在表观遗传水平上的调控机制（例如通过DNA甲基化）知之甚少。在本研究中，我们的目的是比较对照和热应激猪之间的DNA甲基化谱，以确定骨骼肌发育和肉质的候选基因。采用亚硫酸氢盐全基因组测序技术研究了猪背最长肌全基因组DNA甲基化模式。两组在CpG位点的甲基化比例相似，但在非CpG位点的甲基化比例不同。在两组之间总共鉴定了57，147个差异甲基化区域，对应于1，422个差异甲基化基因。基因个体发育和KEGG途径分析表明，这些主要涉及能量和脂质代谢，细胞防御和应激反应，以及钙信号通路。本研究揭示了正常和热应激条件下猪肌肉DNA甲基化的总体模式。本研究结果有助于更好地理解猪肌肉发育和肉质的表观遗传调控。
Heat stress affects muscle development and meat quality in food animals; however, little is known regarding its regulatory mechanisms at the epigenetic level, such as via DNA methylation. In this study, we aimed to compare the DNA methylation profiles between control and heat-stressed pigs to identify candidate genes for skeletal muscle development and meat quality. Whole-genome bisulfite sequencing was used to investigate the genome-wide DNA methylation patterns in the longissimus dorsi muscles of the pigs. Both groups showed similar proportions of methylation at CpG sites but exhibited different proportions at non-CpG sites. A total of 57,147 differentially methylated regions were identified between the two groups, which corresponded to 1,422 differentially methylated genes. Gene ontogeny and KEGG pathway analyses indicated that these were mainly involved in energy and lipid metabolism, cellular defense and stress responses, and calcium signaling pathways. This study revealed the global DNA methylation pattern of pig muscle between normal and heat stress conditions. The result of this study might contribute to a better understanding of epigenetic regulation in pig muscle development and meat quality.