Engineered Diblock Polypeptides Improve DNA and Gold Solubility during Molecular Assembly
Engineered Diblock Polypeptides Improve DNA and Gold Solubility during Molecular Assembly
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DOI：
10.1021/acsnano.6b07291
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发表时间：
2017-01-01
期刊：
影响因子：
17.1
通讯作者：
LaBean, Thomas H.
中科院分区：
文献类型：
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作者：
Estrich, Nicole A.;Hernandez-Garcia, Armando;LaBean, Thomas H.
程序化分子识别正在被开发用于制造用于电子、光子学和医学的有机/无机混合超分子组件的生物纳米制造。例如，基于DNA的纳米技术寻求利用DNA的容易编程的互补碱基配对来指导复杂的、设计的纳米结构的组装。模拟生物系统的最佳溶液条件，可能涉及高浓度的生物大分子(蛋白质、核酸等)。以及各种离子(镁、钠、氯等)的显著浓度。由于需要组装不同的无机成分(金属纳米颗粒、量子点、碳纳米结构等)，要找到同时与所有成分兼容的溶液条件将变得越来越困难。通常，使用化学表面活性剂是不可取的，因此需要开发替代策略。在这里，我们讨论了人工双嵌段多肽在分子组装中作为溶液相容剂的作用。我们描述了两种不同的与DNA有亲和力的双嵌段多肽在稳定DNA折纸和DNA功能化的金纳米粒子(球和棒)中的应用，保护DNA免受酶降解，以及两个三维四面体DNA折纸。我们给出的初始数据表明，二嵌段多肽促进了所需有机/无机组装物在溶液中的形成。
Programmed molecular recognition is being developed for the bionanofabrication of mixed organic/inorganic supramolecular assemblies for applications in electronics, photonics, and medicine. For example, DNA-based nanotechnology seeks to exploit the easily programmed complementary base-pairing of DNA to direct assembly of complex, designed nanostructures. Optimal solution conditions for bionanofabrication, mimicking those of biological systems, may involve high concentrations of biomacromolecules (proteins, nucleic acids, etc.) and significant concentrations of various ions (Mg2+, Na+, Cl-, etc.). Given a desire to assemble diverse inorganic components (metallic nanoparticles, quantum dots, carbon nanostructures, etc.), it will be increasingly difficult to find solution conditions simultaneously compatible with all components. Frequently, the use of chemical surfactants is undesirable, leaving a need for the development of alternative strategies. Herein, we discuss the use of artificial, diblock polypeptides in the role of solution compatibilizing agents for molecular assembly. We describe the use of two distinct diblock polypeptides with affinity for DNA in the stabilization of DNA origami and DNA-functionalized gold nanoparticles (spheres and rods) in solution, protection of DNA from enzymatic degradation, as well as two 3D tetrahedral DNA origamis. We present initial data showing that the diblock polypeptides promote the formation in the solution of desired organic/inorganic assemblies.