Continuous-flow fluorometry of low galactose concentrations in blood or plasma.
Continuous-flow fluorometry of low galactose concentrations in blood or plasma.
复制标题
血液或血浆中低半乳糖浓度的连续流荧光测定。
DOI：
10.1093/clinchem/26.2.282
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发表时间：
1980
影响因子：
9.3
通讯作者：
F. W. Fales
中科院分区：
文献类型：
--
作者：
J. Henderson;F. W. Fales
从血液中清除0-100毫克/升浓度的半乳糖取决于营养肝血流。我们可以通过使用半乳糖氧化酶和过氧化物酶的连续流动方法来测量这种浓度，这在以前是不可能的，后者与荧光底物对羟基苯基乙酸偶联。通过锌/碱沉淀法去除过氧化物酶反应中的干扰物质。使用酶和底物的饱和浓度可使灵敏度最大化。在半乳糖浓度为10、40、70和100 mg/L的血浆样品中，运行内CV值为2.1 ~ 8.6%，日CV值为2.2 ~ 17.2%，其中10 mg/L浓度的CV值最大。正常人清除半乳糖的效率高于中度肝硬化患者。
Clearance of 0-100 mg/L concentrations of galactose from the blood depends on nutrient hepatic blood flow. We can measure such concentrations, which was not previously possible, by a continuous-flow method involving the use of galactose oxidase and peroxidase, the latter being coupled to a fluorogenic substrate, p-hydroxyphenylacetic acid. Interfering substances in the peroxidase reaction are removed by zinc/alkali precipitation. Sensitivity is maximized by using saturating concentrations of the enzymes and substrate. In prepared plasma test samples with galactose concentrations of 10, 40, 70, and 100 mg/L, the within-run CV's ranged from 2.1 to 8.6%, and day-to-day CV's from 2.2 to 17.2%, the largest CV's being for the 10 mg/L concentration. Normal subjects are shown to clear galactose more efficiently than subjects with moderate cirrhosis.