An endothelial receptor for oxidized low-density lipoprotein
An endothelial receptor for oxidized low-density lipoprotein
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DOI：
10.1038/386073a0
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发表时间：
1997-03-06
期刊：
影响因子：
64.8
通讯作者：
Masaki, T
中科院分区：
文献类型：
--
作者：
Sawamura, T;Kume, N;Masaki, T
氧化修饰的低密度脂蛋白（Ox-LDL）引起的内皮功能障碍或活化与动脉粥样硬化的发病机制有关（1-4），其特征为动脉内膜增厚和脂质沉积。Ox-LDL及其脂质成分损害一氧化氮的内皮生成，并诱导白细胞粘附分子和平滑肌生长因子的内皮表达，这可能参与动脉粥样硬化形成（5-7）。培养（8，9）和体内（10，11）中的血管内皮细胞通过假定的受体介导的途径（不涉及巨噬细胞清道夫受体）内化和降解Ox-LDL（12-15）。在这里，我们报告的分子克隆，使用表达克隆策略，从血管内皮细胞的Ox-LDL受体。克隆的受体是一种膜蛋白，在结构上属于C型凝集素家族，并在体内血管内皮和富含血管的器官中表达。
Endothelial dysfunction or activation elicited by oxidatively modified low-density lipoprotein (Ox-LDL) has been implicated in the pathogenesis of atherosclerosis(1-4), characterized by intimal thickening and lipid deposition in the arteries. Ox-LDL and its lipid constituents impair endothelial production of nitric oxide, and induce the endothelial expression of leukocyte adhesion molecules and smooth-muscle growth factors, which may be involved in atherogenesis(5-7). Vascular endothelial cells in culture(8,9) and in vivo(10,11) internalize and degrade Ox-LDL through a putative receptor-mediated pathway that does not involve macrophage scavenger receptors(12-15). Here we report the molecular cloning, using expression cloning strategy, of an Ox-LDL receptor from vascular endothelial cells. The cloned receptor is a membrane protein that belongs structurally to the C-type lectin family, and is expressed in vivo in vascular endothelium and vascular-rich organs.