Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis
Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis
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DOI：
10.1126/science.276.5309.118
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发表时间：
1997-04-04
期刊：
影响因子：
56.9
通讯作者：
Boone, C
中科院分区：
文献类型：
--
作者：
Evangelista, M;Blundell, K;Boone, C
酿酒酵母BNI1基因产物（Bni1p）是一种参与细胞极化、胞质分裂和脊椎动物肢体形成的蛋白质家族成员。在交配信息素的反应，bni1突变体表现出缺陷，在极化形态和重组的基础肌动蛋白细胞骨架，在双杂交实验中，Bni1p形成复合物，与激活形式的Rho相关的鸟苷三磷酸酶Cdc42p，与肌动蛋白，并与两个肌动蛋白相关的蛋白质，profilin和Bud6p（Aip3p）。Bni1p和Bud6p（像Cdc42p和肌动蛋白）定位于交配突起的尖端。Bni1p可能作为一个Cdc42p的目标，连接信息素反应途径的肌动蛋白细胞骨架。
The Saccharomyces cerevisiae BNI1 gene product (Bni1p) is a member of the formin family of proteins, which participate in cell polarization, cytokinesis, and vertebrate limb formation. During mating pheromone response, bni1 mutants showed defects both in polarized morphogenesis and in reorganization of the underlying actin cytoskeleton, In two-hybrid experiments, Bni1p formed complexes, with the activated form of the Rho-related guanosine triphosphatase Cdc42p, with actin, and with two actin-associated proteins, profilin and Bud6p (Aip3p). Both Bni1p and Bud6p (like Cdc42p and actin) localized to the tips of mating projections. Bni1p may function as a Cdc42p target that links the pheromone response pathway to the actin cytoskeleton.