Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm
Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm
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DOI：
10.7554/elife.32041
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发表时间：
2018-01-02
期刊：
影响因子：
7.7
通讯作者：
Blader, Patrick
中科院分区：
文献类型：
--
作者：
Aguillon, Raphael;Batut, Julie;Blader, Patrick
斑马鱼嗅上皮细胞由多种神经元组成，它们被认为具有不同的胚胎起源。例如，虽然纤毛感觉神经元来自前基板外胚层（PPE），但以前的谱系追踪研究表明，促性腺激素释放激素3（Gnrh 3）和微绒毛感觉神经元都来自颅神经嵴（CNC）。我们发现Islet 1/2的表达仅限于与嗅上皮相关的Gnrh 3神经元。然而，出乎意料的是，我们在sox 10突变胚胎中没有发现Islet 1/2+细胞数量的变化，这让人怀疑它们的CNC起源。谱系重建的基础上回溯的时间推移共聚焦数据集，并证实了光转换实验，揭示了Gnrh 3神经元来自前PPE。同样，我们所追踪的所有微绒毛感觉神经元都来自前基板祖细胞。我们的结果表明，细胞类型异质性不是起源于单独的外胚层群体，而是由前基板外胚层内祖细胞的重叠池产生的。
The zebrafish olfactory epithelium comprises a variety of neuronal populations, which are thought to have distinct embryonic origins. For instance, while ciliated sensory neurons arise from preplacodal ectoderm (PPE), previous lineage tracing studies suggest that both Gonadotropin releasing hormone 3 (Gnrh3) and microvillous sensory neurons derive from cranial neural crest (CNC). We find that the expression of Islet1/2 is restricted to Gnrh3 neurons associated with the olfactory epithelium. Unexpectedly, however, we find no change in Islet1/2+ cell numbers in sox10 mutant embryos, calling into question their CNC origin. Lineage reconstruction based on backtracking in time-lapse confocal datasets, and confirmed by photoconversion experiments, reveals that Gnrh3 neurons derive from the anterior PPE. Similarly, all of the microvillous sensory neurons we have traced arise from preplacodal progenitors. Our results suggest that rather than originating from separate ectodermal populations, cell-type heterogeneity is generated from overlapping pools of progenitors within the preplacodal ectoderm.