Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice
Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice
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DOI：
10.1016/j.bbrc.2008.09.132
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发表时间：
2008-12-05
影响因子：
3.1
通讯作者：
Ishigami, Akihito
中科院分区：
文献类型：
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作者：
Kondo, Yoshitaka;Sasaki, Toru;Ishigami, Akihito
维生素C（VC）具有很强的抗氧化作用，在体外能清除超氧自由基。我们通过使用实时成像系统验证了这种性质确实存在于体内，其中光泽精是用于检测衰老标记蛋白-30（SMP 30）/β-内酯酶（GNL）knowkcout（KO）小鼠中超氧化物的荧光探针，该小鼠不能在体内合成VC。对SMP 30/GNL KO小鼠给予1.5 g/L VC [VC（+）] 2、4或8周或拒绝VC [VC（-）]。在第4周和第8周，VC（-）KO小鼠脑中的VC水平分别为
Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knowkcout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were