Developmental differences in susceptibility to neomycin-induced hair cell death in the lateral line neuromasts of zebrafish (Danio rerio)
Developmental differences in susceptibility to neomycin-induced hair cell death in the lateral line neuromasts of zebrafish (Danio rerio)
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DOI：
10.1016/s0378-5955(03)00259-4
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发表时间：
2003-12-01
期刊：
影响因子：
2.8
通讯作者：
Rubel, EW
中科院分区：
文献类型：
--
作者：
Murakami, SL;Cunningham, LL;Rubel, EW
内耳的机械感觉毛细胞在暴露于包括氨基糖苷类抗生素在内的各种药物时容易死亡。在鸟类和哺乳动物的发育过程中，有一段时间对氨基糖苷类诱导的毛细胞死亡相对不敏感。本研究旨在验证斑马鱼(Danio Rerio)对氨基糖苷类诱导的侧线神经肥大毛细胞死亡的敏感性存在发育差异的假说。将不同年龄的斑马鱼仔鱼暴露于不同浓度的新霉素，并用电位活体染料对其毛细胞进行检测，DASPEL结果表明，受精后4天的斑马鱼仔鱼对氨基糖苷类诱导的毛细胞死亡相对不敏感。因此，斑马鱼毛细胞对氨基糖苷类诱导的死亡的敏感性显示出发育差异，类似于鸟类和哺乳动物内耳毛细胞的报道。(C)2003爱思唯尔B.V.保留所有权利。
Mechanosensory hair cells of the inner ear are susceptible to death when exposed to a variety of drugs including aminoglycoside antibiotics. During avian and mammalian development, there is a period of relative insensitivity to aminoglycoside-induced hair cell death. This study was designed to test the hypothesis that zebrafish (Danio rerio) have developmental differences in sensitivity to aminoglycoside-induced hair cell death in the lateral line neuromasts. Larval zebrafish of various ages were exposed to several concentrations of neomycin, and their hair cells were examined using the potentiometric vital dye, DASPEL Results indicate that zebrafish larvae aged 4 days post-fertilization are relatively insensitive to aminoglycoside-induced hair cell death compared to older fish. Thus zebrafish hair cells show developmental differences in sensitivity to aminoglycoside-induced death similar to those reported for inner ear hair cells of birds and mammals. (C) 2003 Elsevier B.V. All rights reserved.