Downregulation of Thy1 in retinal ganglion cells in experimental glaucoma
Downregulation of Thy1 in retinal ganglion cells in experimental glaucoma
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DOI：
10.1080/02713680500545671
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发表时间：
2006-03-01
影响因子：
2
通讯作者：
Grosskreutz, CL
中科院分区：
文献类型：
--
作者：
Huang, W;Fileta, J;Grosskreutz, CL
目的：Thy1是视网膜神经节细胞（retinalganglioncells，RGCs）特有的表面糖蛋白。本研究旨在探讨Thy1在实验性青光眼模型中的表达变化。研究方法：采用体视学计数、Western blot分析、免疫组化、激光捕获显微切割（LCM）及定量PCR方法，观察实验性青光眼视网膜Thy1蛋白及mRNA水平的变化。还进行了视神经挤压后LCM以评估不同损伤后Thy1 mRNA水平。结果如下：眼压升高10天后，RGC数量减少34%，Thy 1蛋白在眼压升高的眼中减少60%，RGC中Thy 1 mRNA水平减少51%。Thy1蛋白和mRNA水平的变化都远大于RGC数量的减少。结论：目前的结果证实，Thy 1 mRNA水平不反映存在的RGC数量，并将其扩展到包括Thy 1蛋白水平的平行降低。这些结果表明，在视网膜损伤模型中，Thy1作为RGC应激的早期标志物，但不是RGC损失的标志物。
Purpose: Thy1 is a surface glycoprotein uniquely expressed in retinal ganglion cells (RGCs) in retina. The aim of this study was to investigate the expression change of Thy1 in a model of experimental glaucoma. Methods: The change of protein and mRNA levels of Thy1 in the retina were studied using stereological counts of back-labeled RGCs, Western blot analysis, immunohistochemistry, and laser capture microdissection (LCM) of RGCs with quantitative PCR analysis of mRNA in a model of experimental glaucoma. LCM after optic nerve crush was also performed to evaluate Thy1 mRNA levels after a different injury. Results: After 10 days of elevated IOP, there was a 34% loss of RGC number, Thy1 protein decreased 60% in eyes with elevated intraocular pressure (IOP), and Thy1 mRNA levels decreased 51% in RGCs. Both protein and mRNA level change of Thy1 is to a much greater extent than RGC number loss. Conclusions: The current results confirm that Thy 1 mRNA levels do not reflect the number of RGCs present and extend this to include a parallel decrease in Thy1 protein levels. These results suggest that Thy1 serves as an early marker of RGC stress, but not a marker of RGC loss, in models of retinal damage.