Phenotypic Variation for Diastatic Power, β-Amylase Activity, and β-Amylase Thermostability vs. Allelic Variation at the Bmy1 Locus in a Sample of North American Barley Germplasm
Phenotypic Variation for Diastatic Power, β-Amylase Activity, and β-Amylase Thermostability vs. Allelic Variation at the Bmy1 Locus in a Sample of North American Barley Germplasm
复制标题
DOI：
10.2135/cropsci2009.04.0231
复制
发表时间：
2010-05-01
期刊：
影响因子：
2.3
通讯作者：
Hayes, P. M.
中科院分区：
文献类型：
--
作者：
Filichkin, T. P.;Vinje, M. A.;Hayes, P. M.
对3种大麦（Hordeum vulgare L.）育种计划试验。我们试图确定因果多态性Bmy1内含子III和编码区用于标记辅助选择。尽管亲本具有相同的Bmy1内含子III和编码区，但春大麦种质中存在丰富的麦芽品质变异。在完成Bmy1测序后，没有多态性与麦芽质量表型相关，表明所观察到的变异的遗传基础存在于Bmy1之外。完整的等位基因测序确定了一个冬大麦亲本，有一个新的Bmy1等位基因（Sd1a）的基础上的氨基酸取代的候选人作为致病因子的表型变异。对Sd1a等位基因的标记辅助选择可以有效地提高糖化力。Sd1a等位基因与低DP相关，并且仅存在于51个品系中的3个品系中，可能是由于前几代被选择用于高DP选择，随后从该育种计划中消除了Sd1a等位基因。这项研究表明，在使用标记辅助选择之前，具有完整的等位基因序列和靶基因功能多态性的知识是重要的。
Malting quality data were collected on malts from three barley (Hordeum vulgare L.) breeding program trials. We tried to identify causal polymorphisms in the Bmy1 intron Ill and coding regions for use in marker-assisted selection. Abundant malting quality variation exists in the spring barley germplasm despite the parents having identical Bmy1 intron Ill and coding regions. After complete Bmy1 sequencing, no polymorphisms associated with malting quality phenotypes, indicating the genetic basis for the observed variation resides outside Bmy1. Complete allele sequencing identified one winter barley parent that had a novel Bmy1 allele (Sd1a) based on amino acid substitutions that are candidates as causative agents for the phenotypic variation. Marker-assisted selection against the Sd1a allele could be effective in improving diastatic power (DP). The Sd1a allele is associated with low DP and is present in only three of the 51 lines, presumably due to preceding generations being selected for high DR Selection for DP has subsequently eliminated the Sd1a allele from this breeding program. This research shows the importance of having complete allele sequences and knowledge of functional polymorphisms in target genes before using marker-assisted selection.