A Simple Optical Coherence Tomography Quantification Method for Choroidal Neovascularization
A Simple Optical Coherence Tomography Quantification Method for Choroidal Neovascularization
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DOI：
10.1089/jop.2015.0049
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发表时间：
2015-10-01
影响因子：
2.3
通讯作者：
Corson, Timothy W.
中科院分区：
文献类型：
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作者：
Sulaiman, Rania S.;Quigley, Judith;Corson, Timothy W.
目的：在激光诱导的脉络膜新生血管(L-CNV)啮齿动物模型中，常规通过使用平板式脉络膜和共聚焦显微镜测量病变大小来评估治疗效果。我们调查了使用椭球体体积测量的光学相干断层扫描(OCT)定量方法是否可与该模型的标准体外评估方法相媲美，以及该方法是否可用于监测与治疗相关的病变变化。方法：用Ar激光打碎C57BL/6J小鼠扩张眼的Bruch膜，玻璃体腔内注射抗血管内皮生长因子(164)或赋形剂，或不注射。用Micron III或InVivoVue系统采集激光后7、10和/或14天的活体OCT图像，计算新生血管损伤体积为椭球体。随后，将病变体积与凝集素染色的脉络膜平面共聚焦Z-STACK图像计算的体积进行比较。结果：从不同成像系统获得的正交二维OCT图像的椭球体体积测量与L新生血管的体外病变体积相关(7、10和14天的Spearman‘s分别为0.82、0.75和0.82)。椭球体积计算可以对抗血管内皮细胞生长因子治疗反应的CNV病变进行时间监测和评估。结论：椭球体体积测量允许快速、定量地使用OCT来评估活体CNV损害。这种新的方法可以用于不同的OCT成像系统，具有区分治疗条件的敏感性。它可作为标准的体外共聚焦定量的有用辅助手段，用于评估临床前CNV模型和其他眼病模型的治疗效果。
Purpose: Therapeutic efficacy is routinely assessed by measurement of lesion size using flatmounted choroids and confocal microscopy in the laser-induced choroidal neovascularization (L-CNV) rodent model. We investigated whether optical coherence tomography (OCT) quantification, using an ellipsoid volume measurement, was comparable to standard ex vivo evaluation methods for this model and whether this approach could be used to monitor treatment-related lesion changes. Methods: Bruch's membrane was ruptured by argon laser in the dilated eyes of C57BL/6J mice, followed by intravitreal injections of anti-VEGF(164) or vehicle, or no injection. In vivo OCT images were acquired using Micron III or InVivoVue systems at 7, 10, and/or 14 days post-laser and neovascular lesion volume was calculated as an ellipsoid. Subsequently, lesion volume was compared to that calculated from confocal Z-stack images of agglutinin-stained choroidal flatmounts. Results: Ellipsoid volume measurement of orthogonal 2-dimensional OCT images obtained from different imaging systems correlated with ex vivo lesion volumes for L-CNV (Spearman's =0.82, 0.75, and 0.82 at days 7, 10, and 14, respectively). Ellipsoid volume calculation allowed temporal monitoring and evaluation of CNV lesions in response to antivascular endothelial growth factor treatment. Conclusions: Ellipsoid volume measurements allow rapid, quantitative use of OCT for the assessment of CNV lesions in vivo. This novel method can be used with different OCT imaging systems with sensitivity to distinguish between treatment conditions. It may serve as a useful adjunct to the standard ex vivo confocal quantification, to assess therapeutic efficacy in preclinical models of CNV, and in models of other ocular diseases.