Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation
Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation
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DOI：
10.1016/j.biortech.2012.01.125
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发表时间：
2012-04-01
影响因子：
11.4
通讯作者：
Torpee, Salwa
中科院分区：
文献类型：
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作者：
Cheirsilp, Benjamas;Torpee, Salwa
在光合自养、异养和兼养培养条件下，筛选出能够积累高脂肪含量的微藻。与光自养和异养培养相比，混合营养培养中所有供试菌株的生物量和脂质产量均显著提高。其中，海洋小球藻和微拟球藻具有较高的油脂产量，是生物柴油生产的理想候选菌株。研究了光照强度和初始葡萄糖浓度对两株菌生长和油脂含量的影响。虽然增加光照强度和初始葡萄糖浓度增强了两种菌株的生长，但降低了它们的脂质含量。为了产生具有高脂质含量的大量生物质，进行具有逐步增加的光强度的补料分批培养。通过这种策略的脂质产量约为常规分批培养的两倍。两种微藻的脂肪酸组成均以C16 ~ C18为主（>80%），适合生物柴油的生产。(C)2012爱思唯尔有限公司保留所有权利。
Microalgae capable of accumulating high lipid content were screened in photoautotrophic, heterotrophic and mixotrophic cultures. The biomass and lipid production of all tested strains in mixotrophic culture were notably enhanced in comparison with photoautotrophic and heterotrophic cultures. Among the tested strains, marine Chlorella sp. and Nannochloropsis sp. were ideal candidates for biodiesel production because of their high lipid production. The influence of light intensity and initial glucose concentration on growth and lipid content of both strains were investigated. Although increasing light intensity and initial glucose concentration enhanced the growth of both strains, it reduced their lipid content. To produce a high amount of biomass with high lipid content, a fed-batch cultivation with stepwise increasing light intensity was performed. The lipid production by this strategy was approximately twice that of conventional batch cultivation. The main fatty acid compositions of the two microalgae were C16-C18 (>80%) which are appropriate for biodiesel production. (C) 2012 Elsevier Ltd. All rights reserved.