Detection of the Presence of Gold Nanoparticles in Organs by Transmission Electron Microscopy.
Detection of the Presence of Gold Nanoparticles in Organs by Transmission Electron Microscopy.
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DOI：
10.3390/ma3094681
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发表时间：
2010-09-27
期刊：
影响因子：
--
通讯作者：
Geertsma RE
中科院分区：
文献类型：
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作者：
Jong WH;Burger MC;Verheijen MA;Geertsma RE
将10 nm和250 nm的金纳米颗粒静脉注射给大鼠。给药后24小时，收集组织并准备用于透射电子显微镜（TEM）。在用 10 nm 金纳米粒子处理的动物的肝脏和脾脏中，观察到纳米粒子群，通过能量色散 X 射线 (EDX) 分析可以肯定地识别出含有金，而在心脏、肾脏和大脑中却无法检测到纳米粒子。 10 nm 金纳米颗粒存在于网状内皮系统 (RES) 的吞噬细胞中。在所研究的任何器官中均未检测到 250 nm 金纳米颗粒。考虑到所施用的 250 nm 金纳米颗粒的数量，计算表明，鉴于理论上存在于一个 TEM 组织切片中的颗粒数量非常少，实际上几乎不可能检测到 TEM 制剂中的 250 nm 金纳米颗粒。这表明需要施用相对大量的纳米颗粒才能通过 TEM 检测器官中的纳米颗粒。在许多样本中，在肝细胞和大脑血管内皮中发现了几个与预期大小大致相同的球状结构。然而，EDX 检测的元素分析表明这些结构不含金。因此，我们的研究表明，纳米粒子的体内鉴定不能仅依赖于细胞中纳米结构的检测。为了对纳米材料进行积极的鉴定，需要对纳米材料的组成元素进行额外的鉴定。
Gold nanoparticles of 10 nm and 250 nm were intravenously injected in rats. At 24 h after administration, tissues were collected and prepared for transmission electron microscopy (TEM). In the liver and spleen of animals treated with 10 nm gold nanoparticles, groups of nanoparticles were observed that could be positively identified by Energy Dispersive X-ray (EDX) analysis to contain gold, while nanoparticles could not be detected in the heart, kidney and brain. The 10 nm gold nanoparticles were present in the phagocytic cells of the reticulo-endothelial system (RES). The 250 nm gold nanoparticles could not be detected in any of the organs investigated. Considering the number of 250 nm gold nanoparticles administered, calculations showed that it would indeed be almost impossible to detect the 250 nm gold nanoparticles in TEM preparations in view of the very low number of particles that would be theoretically present in one TEM tissue section. This shows that relatively high numbers of nanoparticles need to be administered to enable the detection of nanoparticles in organs by TEM. In a number of samples, several globular structures of approximately the expected size were found in liver cells and the endothelium of blood vessels in the brain. However, elemental analysis with EDX detection showed that these structures did not contain gold. Our studies thus indicate that the in vivo identification of nanoparticles cannot only depend on the detection of nanosized structures in cells. An additional identification of the composing elements of the nanomaterial is necessary for a positive identification of the nanomaterial.