Selective transcription factor induction in retinal pigment epithelial cells during photoreceptor phagocytosis
Selective transcription factor induction in retinal pigment epithelial cells during photoreceptor phagocytosis
复制标题
DOI：
10.1074/jbc.271.45.28458
复制
发表时间：
1996-11-08
影响因子：
4.8
通讯作者：
Bazan, NG
中科院分区：
文献类型：
--
作者：
Ershov, AV;Lukiw, WJ;Bazan, NG
在原代细胞培养物中研究了正常 Long Evans 和皇家外科医生学院-rdy(+)p(+)大鼠和营养不良皇家外科医生学院-p(+)大鼠视网膜色素上皮细胞在视杆外节吞噬过程中早期反应基因的表达。 Northern分析显示，正常视网膜色素上皮细胞中zif-268 (egr-1)、c-fos和tis-1 (NGF1-B) mRNA的丰度在视杆外节吞噬过程中迅速而短暂地增加，但在吞噬乳胶颗粒过程中则没有。在接受视杆细胞外节攻击的英国皇家外科学院 p(+) 营养不良性视网膜色素上皮细胞中，未发现基因表达增加。如电泳迁移率变动测定所示，使用源自视杆外节攻击的对照视网膜色素上皮细胞的核蛋白提取物和 zif-268、AP-1、AP-2 或 tis-1 共有寡核苷酸检测到凝胶迁移带强度的显着短期增加。当使用核因子KB共有寡核苷酸或在研究的时间过程中测量早期反应基因前列腺素H合酶2 mRNA时，没有检测到这种增加。结果表明，在视网膜色素上皮细胞中，rad外节特异性吞噬作用伴随着编码转录因子的早期反应基因的选择性表达。预计这些转录因子诱导的特定模式将调节基因级联的表达。
Expression of early response genes during rod outer segment phagocytosis by normal Long Evans and Royal College of Surgeons-rdy(+)p(+) rats and by dystrophic Royal College of Surgeons-p(+) rat retinal pigment epithelial cells was studied in primary cell culture. Northern analysis revealed that the abundance of zif-268 (egr-1), c-fos, and tis-1 (NGF1-B) mRNA was rapidly and transiently increased in normal retinal pigment epithelial cells during rod outer segment phagocytosis but not during phagocytosis of latex particles. No increase in gene expression was found in Royal College of Surgeons-p(+) dystrophic retinal pigment epithelial cells challenged with rod outer segments. As shown by electrophoretic mobility shift assay, a prominent short term increase in the intensity of the gel-shifted band was detected using nuclear protein extracts derived from rod outer segment-challenged, control retinal pigment epithelial cells and zif-268, AP-1, AP-2, or tis-1 consensus oligonucleotides. No such increase was detected when using nuclear factor KB consensus oligonucleotide or when the early response gene prostaglandin H synthase-2 mRNA was measured over the time course studied. The results suggest that in retinal pigment epithelial cells, rad outer segment-specific phagocytosis is accompanied by the selective expression of early response genes coding for transcription factors. The specific pattern of the induction of these transcription factors is predicted to modulate the expression of gene cascades.