Variation in gene expression profiles of peripheral blood mononuclear cells from healthy volunteers
Variation in gene expression profiles of peripheral blood mononuclear cells from healthy volunteers
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DOI：
10.1152/physiolgenomics.00080.2005
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发表时间：
2005-08-11
影响因子：
4.6
通讯作者：
Elliott, RM
中科院分区：
文献类型：
--
作者：
Eady, JJ;Wortley, GM;Elliott, RM
健康人体组织基因转录谱的个体内和个体间变异的正常程度尚未得到广泛研究。在这里描述的研究中，微阵列被用来分析外周血单个核细胞中的基因转录，这些细胞是从表面健康的志愿者中以每周为间隔获得的连续血液样本中制备的。发现所检查的大多数基因的转录水平在来自单个供体的样品内显著一致。相反，在从不同供体获得的样品中观察到显著差异。根据性别、年龄、体重指数和不同比例的不同白细胞亚群的存在，确定了表现出差异表达的基因。这些结果强调了遗传和环境因素的重要贡献，以及不同细胞类型的不同代表性，在确定人体组织的整体基因转录谱。然而，这项研究也提供了证据，表明在一个人体内，取样组织的基因转录谱可以随着时间的推移而相对稳定。
The normal degree of intra- and interindividual variation in gene transcription profiles of healthy human tissues has not been extensively investigated. In the study described here, microarrays were employed to analyze gene transcription in peripheral blood mononuclear cells prepared from serial blood samples that had been obtained, at weekly intervals, from apparently healthy human volunteers. Transcript levels for the majority of genes examined were found to be remarkably consistent within samples from a single donor. Conversely, marked differences were observed in samples obtained from different donors. Genes that exhibited differential expression dependent on sex, age, body mass index, and the presence of varying proportions of different leukocyte subsets were identified. These results emphasize the important contributions of genetic and environmental factors, as well as varying representation of different cell types, in determining the overall gene transcriptional profiles of human tissues. However, the study also provides evidence that, within an individual, the gene transcription profiles of sampled tissues can be comparatively stable over time.