Overexpression of the transcription factor HvSNAC1 improves drought tolerance in barley (Hordeum vulgare L.)
Overexpression of the transcription factor HvSNAC1 improves drought tolerance in barley (Hordeum vulgare L.)
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DOI：
10.1007/s11032-013-9958-1
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发表时间：
2014-02-01
影响因子：
3.1
通讯作者：
Harwood, W. A.
中科院分区：
文献类型：
--
作者：
Al Abdallat, A. M.;Ayad, J. Y.;Harwood, W. A.
NAC蛋白是一类植物特异性转录因子家族，参与许多植物细胞过程，包括对非生物胁迫的应答。在这项研究中，一个cDNA克隆编码的HvSNAC1转录因子是从干旱胁迫大麦分离使用生物信息学方法的基础上的氨基酸序列数据的压力相关的SNAC1蛋白从水稻。HvSNAC1蛋白的氨基酸序列分析表明，该蛋白属于NAC蛋白的应激进化枝，包括SNAC1和TaNAC2。表达分析表明，HvSNAC1基因强烈诱导不同的非生物胁迫，包括干旱。在组成型启动子的控制下，在大麦中过表达HvSNAC1产生的植物与野生型植物相比，在良好的浇水条件下正常生长。与野生型相比，过表达HvSNAC1的转基因大麦在不同的生长阶段表现出更高的耐旱性。此外，组成型过表达HvSNAC1导致改善水分状况，光合活性和减少水分流失率相比，野生型植物在干旱条件下。此外，转基因植物还显示出显著提高的生产力，如在严重的田间干旱条件下生物产量比野生型植物增加所反映的。总之，HvSNAC1基因可能是一个有用的工具，用于提高大麦生产力在田间干旱条件下，在正常田间条件下的生长没有损害。
NAC proteins constitute a family of plant-specific transcription factors that are involved in many plant cellular processes including responses to abiotic stress. In this study, a cDNA clone encoding the HvSNAC1 transcription factor was isolated from drought-stressed barley using a bioinformatics approach based on amino acid sequence data of the stress-related SNAC1 protein from rice. Phylogenetic analysis of the deduced amino acid sequence of HvSNAC1 showed that this protein belongs to the stress clade of NAC proteins that include SNAC1 and TaNAC2. Expression analysis indicated that the HvSNAC1 gene is strongly induced by different abiotic stresses including drought. Overexpression of HvSNAC1 in barley under the control of a constitutive promoter produced plants that grew normally under well-watered conditions when compared with wild-type plants. Transgenic barley plants overexpressing HvSNAC1 showed higher drought tolerance at different growth stages when compared with wild-type plants. In addition, the constitutive overexpression of HvSNAC1 resulted in improved water status, photosynthetic activity and reduced water loss rate when compared with wild-type plants under drought conditions. Furthermore, the transgenic plants also showed significantly improved productivity, as reflected by the increase in biological yield over the wild-type plants under severe field drought conditions. In conclusion, the HvSNAC1 gene could be a useful tool for improving barley productivity under field drought conditions without impairment in growth under normal field conditions.