Gene Characterization and Expression Analysis Reveal the Importance of Auxin Signaling in Bud Dormancy Regulation in Tea Plant
Gene Characterization and Expression Analysis Reveal the Importance of Auxin Signaling in Bud Dormancy Regulation in Tea Plant
复制标题
基因表征和表达分析揭示了生长素信号在茶树芽休眠调节中的重要性
DOI：
10.1007/s00344-018-9834-7
复制
发表时间：
2019-03-01
影响因子：
4.8
通讯作者：
Wang, Xinchao
中科院分区：
文献类型：
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作者：
Hao, Xinyuan;Tang, Hu;Wang, Xinchao
茶树是一种重要的经济木本植物，其原叶用于茶叶生产。冬芽休眠不仅是茶树生存的生物学策略，也是影响茶叶生产经济效益的生物学事件。基于我们先前对不同休眠状态下腋芽的转录组分析，我们重新分析了大量差异表达的生长素相关基因，并确定了生长素信号在茶树芽休眠调节中的相对重要性。随后，我们克隆了AUX/LAX、PIN/PILS、AUX/IAA、GH3和SAUR基因家族中几个生长素相关基因的全长cDNA序列，对这些基因进行了特征分析和系统发育分析，并利用它们编码的蛋白序列进行了保守的基序搜索。结合IAA含量检测，对越冬芽在活跃-休眠-活跃状态转换过程中的组织特异性表达和时程表达进行了分析。大体上，生长素相关基因的表达模式与芽中IAA含量的变化及其活跃-休眠状态的转变是一致的。特别是，我们证实了生长素运输基因CsLAX2和早期生长素反应基因CsGH3.6、CsGH3.9、CsGH3.10、CsIAA26、CsIAA33、CsSAUR50和CsSAUR41在茶树芽休眠调节中的重要作用。我们的结果验证了生长素在茶树休眠调节中的重要作用，并为进一步的功能研究提供了有用的信息。
The tea plant is an economically important woody plant whose raw leaves are used for tea production. Winter bud dormancy is not only a useful biological strategy for tea plant survival but also a biological event that affects the economics of tea production. Based on our previous transcriptome analysis of axillary buds in different dormancy states, we reanalyzed a large number of differentially expressed auxin-related genes and determined the relative importance of the roles of auxin signaling in bud dormancy regulation in tea plant. Subsequently, we cloned the full-length cDNA sequence of several auxin-related genes in the AUX/LAX, PIN/PILS, AUX/IAA, GH3, and SAUR gene families, characterized these genes and performed a phylogenetic analysis, and conserved motif search using the sequences of their encoded proteins. Expression profile analyses, including tissue-specific expression and time-course expression during the active-dormant-active status transitions of overwinter buds, were carried out, combined with IAA content detection. Generally, the expression patterns of auxin-related genes were consistent with the IAA content changes in buds and their active-dormant status transition. In particular, we confirmed the crucial roles of the auxin transport geneCsLAX2and the early auxin response genesCsGH3.6, CsGH3.9, CsGH3.10, CsIAA26, CsIAA33, CsSAUR50, andCsSAUR41in bud dormancy regulation in tea plant. Our results validate the important role of auxin in tea plant dormancy regulation and provide useful information for further functional studies.