Solution structure of the Trp operator of Escherichia coli determined by NMR.
Solution structure of the Trp operator of Escherichia coli determined by NMR.
复制标题
通过 NMR 测定大肠杆菌色氨酸操纵子的溶液结构。
DOI：
10.1021/bi00390a029
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Jardetzky,O
中科院分区：
文献类型：
--
作者：
Lefèvre,JF;Lane,AN;Jardetzky,O
Jean-Francois Lefevre, 1 Andrew N. Lane, § and Oleg Jardetzky** Stanford Magnetic Resonance Laboratory, Stanford University, Stanford, California 94305-5055 Received November 18, 1986; Revised Manuscript Received March 9, 1987 abstract: We have assigned the majority of the nonexchangeable protons in the NMR spectrum of the 20 base-pair fragment of DNA corresponding to the Trp operator of Escherichia coli. The sequence (CGTACTAGTTAACTAGTACG) also contains a Pribnow box (underlined). Variation of the intrinsic spin-lattice relaxation rate constants of the H8's along the sequence indicates that the structure of the oligonucleotide is not regular. Splitting patterns of the HI'resonances in the deoxyriboses, obtained from a two-dimensional/-resolved experiment, allowed the dominant pucker mode of each nucleotide to be determined. Intranucleotide NOEs from the sugar protons HI', H2', and H3'to the base protons were used todetermine the conformation of each nucleotide (puckers and glycosidic torsion angles). The relative orientations of nucleotide units (roll, propeller twist, helical twist angle, and pitch) were calculated by using internucleotide NOEs between protons of neighboring nucleotides in the sequence. All these parameters were determined for each step along the 20-mer. The structure belongs to the B family of conformations, but variations of the local geometry are observed from step to step. Some of the variations, such as the roll and the twist angles, can be predicted by the rules of Calladme and Dickerson [Calladme, C. R., & Dickerson, R. E.(1983)/. Mol. Biol. 166, 419-441], Thepuckers of the deoxyriboses of purines are found mainly in conformations near C2'endo, while those of the pyrimidines preferC3'endo and related con-formations. Glycosidic torsion angles obtained for purines are larger than those of pyrimidines. Except for this last observation, the general properties of the operator DNA structure are comparable with those of crystal structures of B DNA of other sequences.
