Involvement of calcium in interactions between gingival epithelial cells and Porphyromonas gingivalis
Involvement of calcium in interactions between gingival epithelial cells and Porphyromonas gingivalis
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DOI：
10.1111/j.1574-6968.1996.tb08521.x
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发表时间：
1996-11-01
影响因子：
2.1
通讯作者：
Lamont, RJ
中科院分区：
文献类型：
--
作者：
Izutsu, KT;Belton, CM;Lamont, RJ
牙龈卟啉单胞菌是一种牙周病原菌，可侵袭原代培养的牙龈上皮细胞。山茶素和1，2-二(2-氨基苯氧基)乙烷-N，N，N‘，N’-四乙酸乙酰氧甲酯(BAPTA/AM)可显著抑制这种侵袭(74-81%)，而EDTA和阿米洛利则不能。因此，细胞内钙离子的释放和随后的胞浆[Ca~(2+)]增加可能参与了侵袭过程，而Ca~(2+)内流则不参与。此外，约30%的牙龈上皮细胞在急性暴露于牙龈假单胞菌时胞浆[Ca~(2+)]短暂升高，但在未暴露的细胞或暴露于非侵袭性大肠杆菌的细胞中则没有。这些发现表明，牙龈假单胞菌侵袭上皮细胞与激活依赖于[Ca~(2+)]的宿主细胞信号系统有关。
Porphyromonas gingivalis, a periodontal pathogen, can invade primary cultures of gingival epithelial cells. This invasion was significantly inhibited (74-81%) by thapsigargin and 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, acetoxy-methyl ester (BAPTA/AM), but not by EDTA or amiloride. Release of Ca2+ from an intracellular store and the subsequent increase in cytosolic [Ca2+] may, therefore, be involved in the invasion process, while Ca2+ influx is not. Moreover, cytosolic [Ca2+] was found to increase transiently in about 30% of gingival epithelial cells acutely exposed to P. gingivalis, but not in unexposed cells, or in cells exposed to noninvasive Escherichia coli. These findings indicate that P. gingivalis invasion of epithelial cells is correlated with activation of [Ca2+]-dependent host cell signaling systems.