Novel genetic variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a novel Ehrlichia sp in wild deer and ticks on two major islands in Japan
Novel genetic variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a novel Ehrlichia sp in wild deer and ticks on two major islands in Japan
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DOI：
10.1128/aem.72.2.1102-1109.2006
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发表时间：
2006-02-01
影响因子：
4.4
通讯作者：
Tajima, T
中科院分区：
文献类型：
--
作者：
Kawahara, M;Rikihisa, Y;Tajima, T
野鹿是多种埃里希体和无形体的重要天然宿主之一，这些埃里希体和无形体在美国和欧洲引起人类埃里希体病或无形体病。本研究的主要目的是确定埃利希体和无形体是否以及哪些种类自然感染日本的鹿。通过 PCR 检测和 16S rRNA 基因、主要外膜蛋白 p44 基因和 groESL 测序，检测从日本两个主要岛屿（北海道和本州）野鹿采集的血液样本中是否存在埃里希体和无形体。在 126 只野鹿中的 33 只 (26%) 中鉴定出了代表埃里希体和无形体的 4 个物种和两个属的 DNA。 DNA 序列分析揭示了鹿血液样本中存在新型嗜吞噬细胞无形体、新型埃利希体、中央无形体和牛无形体。以前在鹿身上都没有发现过这些。新埃利希体 sp.,A.在本州岛的 Hemaphysalis longicornis 蜱中也检测到了牛和 A.centrale。这些结果表明，在日本的野鹿和蜱中已经建立了与美国或欧洲发现的埃利希体和无形体物种不同的地方性动物循环。
Wild deer are one of the important natural reservoir hosts of several species of Ehrlichia and Anaplasma that cause human ehrlichiosis or anaplasmosis in the United States and Europe. The primary aim of the present study was to determine whether and what species of Ehrlichia and Anaplasma naturally infect deer in Japan. Blood samples obtained from wild deer on two major Japanese islands, Hokkaido and Honshu, were tested for the presence of Ehrlichia and Anaplasma by PCR assays and sequencing of the 16S rRNA genes, major outer membrane protein p44 genes, and groESL. DNA representing four species and two genera of Ehrlichia and Anaplasma was identified in 33 of 126 wild deer (26%). DNA sequence analysis revealed novel strains of Anaplasma phagocytophilum, a novel Ehrlichia sp., Anaplasma centrale, and Anaplasma bovis in the blood samples from deer. None of these have been found previously in deer. The new Ehrlichia sp.,A. bovis, and A. centrale were also detected in Hemaphysalis longicornis ticks from Honshu Island. These results suggest that enzootic cycles of Ehrlichia and Anaplasma species distinct from those found in the United States or Europe have been established in wild deer and ticks in Japan.