Classic and non-classic progesterone receptors are both expressed in human spermatozoa
Classic and non-classic progesterone receptors are both expressed in human spermatozoa
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DOI：
10.1055/s-2005-861023
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发表时间：
2005-01-01
影响因子：
2.2
通讯作者：
Falkenstein, E
中科院分区：
文献类型：
--
作者：
Lösel, R;Breiter, S;Falkenstein, E
孕酮是哺乳动物精子顶体反应的生理诱导剂之一。对孕酮有反应的受体尚未确定，其性质在许多方面与经典的核孕酮受体的性质不同，这表明有一种新的或非经典的受体参与。在这项研究中，我们研究了一种新型孕酮结合蛋白（hmPR 1/PGMRC 1）及其直系同源物（hmPR 2/PGMRC 2）的表达，这些蛋白此前已在肝微粒体中鉴定，并被认为是受体候选蛋白，沿着的是核孕酮受体。对纯化过程进行了优化，特别强调了对单个供体样本中白细胞污染的控制。结果表明，所有三种蛋白质都在人类精子中表达，分别在46%、42%和37.5%的个体样本中检测到转录本（n = 24）。
Progesterone is one of the physiological inducers of the acrosome reaction in mammalian spermatozoa. The receptor that responds to progesterone is not yet identified, and its properties differ in many aspects from the properties of the classic nuclear progesterone receptor, suggesting the participation of a novel or nonclassic receptor. In this study, we investigated the expression of a novel progesterone-binding protein (hmPR1/PGMRC1) and its ortholog (hmPR2/PGMRC2), which have previously been identified in liver microsomes and are considered receptor candidates, along with the nuclear progesterone receptor. The purification procedure was optimized with special emphasis on the control of leukocyte contamination in single donor samples. The results indicate that all three proteins are expressed in human sperm, as transcripts have been detected in 46%, 42% and 37.5% of individual samples, respectively (n = 24).