Site directed substitution of 5-hydroxymethyluracil for thymine in replicating phi X-174am3 DNA via synthesis of 5-hydroxymethyl-2'-deoxyuridine-5'-triphosphate.
Site directed substitution of 5-hydroxymethyluracil for thymine in replicating phi X-174am3 DNA via synthesis of 5-hydroxymethyl-2'-deoxyuridine-5'-triphosphate.
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通过合成 5-羟甲基-2-脱氧尿苷-5-三磷酸，在复制 phi X-174am3 DNA 中用 5-羟甲基尿嘧啶定点取代胸腺嘧啶。
DOI：
10.1093/nar/19.12.3337
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发表时间：
1991
影响因子：
14.9
通讯作者：
Teebor,GW
中科院分区：
文献类型：
--
作者：
Levy,DD;Teebor,GW
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5-hydroxymethyluracil (HmUra) is formed in DNA as a product of oxidatlve attack on the methyl group of Thy. It Is removed from DNA by HmUra-DNA glycosylase. To determine whether the replacement of Thy by HmUra is mutagenic, which might explain the repairabillty of HmUra, a HmUra residue was substituted for Thy In a target (amber) codon byin vitroextension of an oligonucleotlde primer annealed to øX-174am3virion DNA. This was accomplished by synthesizing HmdUTP and using DNA polymerase to effect primer extension.E.collspheroplasts were transfected with the HmUra-containlng DNA and the yield of revertant phage determined following replication In the bacterial host. SinceE. colido not express HmUra-DNA glycosylase activity, mutagenesis could be assessed In the absence of repair. X2canalysis showed that replacing Thy with HmUra did not result in an Increase In revertant phage. These data indicate that the oxidation of Thy to HmUra in cellular DNA probably does not result in substantial mutagenesis.
核酸的非线性激光光物理学、光化学和光生物学
DOI：
--
发表时间：
1983
期刊：
影响因子：
--
作者：
D. N. Nikogosyan;V. Letokhov
通讯作者：
V. Letokhov