Induction of embryogenic tissue and maturation of somatic embryos in Pinus brutia TEN
Induction of embryogenic tissue and maturation of somatic embryos in Pinus brutia TEN
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DOI：
10.1007/s11240-006-9137-8
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发表时间：
2006-08
期刊：
影响因子：
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通讯作者：
Tolga Yildirim;Z. Kaya;K. Isik
中科院分区：
文献类型：
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作者：
Tolga Yildirim;Z. Kaya;K. Isik
土耳其赤松（Pinus brutiaTEN）的胚胎发生组织（ET）起源于从15棵不同优树中取样的未成熟前子叶合子胚。从2003年6月10日至7月22日，每周收集7次。DCR培养基中添加13.6μM 2，4-二氯苯氧乙酸（2，4-D）和2.2μM苄氨基嘌呤（BAP），用于ET的启动和维持。研究中ET的总体起始频率为11.6%，每棵树的起始率范围为4.7%至24.1%。在检测的12，940个外植体中，3.4%在5次传代培养后转化为已建立的细胞系（ECL）。结果表明，80μM阿坝、3%蔗糖、6%麦芽糖和3.75%PEG +1%结冷胶的组合对体细胞胚的成熟效果最好。
Embryogenic tissues (ET) of Turkish red pine (Pinus brutiaTEN) were initiated from immature precotyledonary zygotic embryos sampled from 15 different plus trees. Seven collections were made weekly from June 10 to July 22, 2003. DCR basal medium supplemented with 13.6μM 2,4–dichlorophenoxyacetic acid (2,4-D) and 2.2μM benzylaminopurine (BAP) was used for initiation and maintenance of ET. Overall initiation frequency of ET in the study was 11.6%, initiation rates ranging between 4.7% and 24.1% per tree. Out of 12,940 explants tested, 3.4% were converted into established cell lines (ECL) following five subcultures. Of the maturation treatments tested, 80μM ABA, sucrose (3%) and maltose (3% and 6%), and 3.75% PEG combined with 1% gellan gum were the most suitable combination for somatic embryo maturation.