Encapsulation of Lactic Acid Bacteria in Colloidosomes
Encapsulation of Lactic Acid Bacteria in Colloidosomes
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DOI：
10.1021/la303043n
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发表时间：
2012-11-20
期刊：
影响因子：
3.9
通讯作者：
Routh, Alexander F.
中科院分区：
文献类型：
--
作者：
Keen, Polly H. R.;Slater, Nigel K. H.;Routh, Alexander F.
制备包封活乳酸菌的聚合物胶体体。将153 nm聚（甲基丙烯酸甲酯-共-丙烯酸丁酯）胶乳颗粒加上卷曲乳杆菌的水性悬浮液在向日葵油的连续相中乳化。通过向油相中加入少量的乙醇和盐，乳液液滴表面的胶乳颗粒聚集形成胶体体壳。已使用光学，共聚焦和扫描电子显微镜检查胶体体。使用荧光分子探针测试细菌的活力。包封的乳酸菌能够从溶液中代谢葡萄糖并产生酸，尽管与未包封的微生物相比速率较慢。这证明了通过胶体体壳的运输限制和由于包封引起的细胞代谢的限制。也证明了通过在胶体体中封装来保护细菌;与未封装的微生物相比，显著更大数量的封装细菌在模拟胃条件下保持活力。
Polymeric colloidosomes encapsulating viable lactic acid bacteria were prepared. An aqueous suspension of 153 nm poly(methyl methacrylate-co-butyl acrylate) latex particles plus Lactobacillus crispatus was emulsified in a continuous phase of sunflower oil. By adding a small amount of ethanol and salt to the oil phase, the latex particles at the surface of the emulsion droplets aggregate to form the colloidosome shells. The colloidosomes have been examined using optical, confocal, and scanning electron microscopies. The viability of the bacteria was tested using fluorescent molecular probes. The encapsulated lactic acid bacteria were able to metabolize glucose from solution and produce acid albeit at a slower rate compared to unencapsulated microbes. This demonstrates transport limitation through the colloidosome shell and restriction of the cellular metabolism due to encapsulation. Protection of the bacteria by encapsulation in colloidosomes was also demonstrated; a significantly larger number of encapsulated bacteria maintained viability in simulated stomach conditions compared to unencapsulated microbes.