Involvement of antisense RNA in replication control of the lactococcal plasmid pND324
Involvement of antisense RNA in replication control of the lactococcal plasmid pND324
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DOI：
10.1111/j.1574-6968.1998.tb13118.x
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发表时间：
1998-07-15
影响因子：
2.1
通讯作者：
Dunn, NW
中科院分区：
文献类型：
--
作者：
Duan, KM;Liu, CQ;Dunn, NW
pND324 属于乳酸乳球菌密切相关的 theta 型质粒家族。鉴定出一种反义 RNA，称为反转录 (ctRNA)，它与编码 RepB 的 mRNA 前导序列互补，RepB 是质粒复制所必需的蛋白质。当ctRNA的合成通过其启动子区域内的定点诱变被废除时，突变复制子的拷贝数增加了1.8倍。在其他 12 个已发表的乳球菌 theta 型质粒中可以很容易地识别出类似的 ctRNA 启动子序列，这表明它们都编码类似的 ctRNA 介导的调节机制。 (C) 1998 年由 Elsevier Science B.V. 出版。保留所有权利。
pND324 belongs to a family of closely related theta-type plasmids from Lactococcus lactis. An antisense RNA, termed countertranscript (ctRNA), was identified which is complementary to the leader sequence of the mRNA that encodes RepB, a protein essential for plasmid replication. When the synthesis of ctRNA was abolished by site-directed mutagenesis within its promoter region, the mutant replicon showed a 1.8-fold increase in copy number. Similar ctRNA promoter sequences are readily identifiable in 12 other published lactococcal theta-type plasmids, suggesting that they all encode a similar ctRNA-mediated regulatory mechanism. (C) 1998 Published by Elsevier Science B.V. All rights reserved.