Dietary protein content affects isotopic carbon and nitrogen turnover.
Dietary protein content affects isotopic carbon and nitrogen turnover.
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膳食蛋白质含量影响同位素碳和氮的周转
DOI：
10.1002/rcm.6737
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Schnyder H
中科院分区：
文献类型：
--
作者：
Braun A;Auerswald K;Vikari A;Schnyder H
同位素周转率量化了器官和排泄物中元素的代谢更新过程。了解动物器官和排泄物的同位素周转对于通过稳定同位素分析重建饮食是必要的，如用于动物生态学，古生物学和食品认证。饲粮蛋白质含量对碳氮同位素周转的影响对于大多数哺乳动物的器官和排泄物来说，延迟（即，代表摄入和更新开始之间的时间以及半衰期）是未知的。（延迟和周转率），我们给18只大鼠喂食蛋白质维持或高于蛋白质维持的饮食，并量化了它们十个器官和排泄物中同位素碳和氮的周转。这些包括排泄物粪便和尿液，内脏器官血浆，肝，肾，肺和脾，脑组织脑，和肌肉组织心脏和肌肉。对于数据分析，我们使用分段线性/非线性指数模型，可以同时量化延迟和周转率。碳和氮周转的延迟约为0.5天，不受膳食蛋白质含量的影响。以下反应进展期间的半衰期范围为1 - 45天，从排泄物到内脏器官再到肌肉和大脑器官逐渐增加。高蛋白质喂养的大鼠氮半衰期缩短30%，碳半衰期缩短20%。结论器官和排泄物的更新时间取决于膳食蛋白质含量。因此，同位素饮食重建面临着相同器官或排泄物中半衰期的变化，改变了可以感知信息的时间窗口。版权所有© 2013约翰威利父子有限公司.
RATIONALEIsotopic turnover quantifies the metabolic renewal process of elements in organs and excreta. Knowledge of the isotopic turnover of animal organs and excreta is necessary for diet reconstruction via stable isotope analysis, as used in animal ecology, palaeontology and food authentication. Effects of dietary protein content on the isotopic carbon and nitrogen turnover (i.e. delay, representing the time between ingestion and start of renewal, and half‐life) are unknown for most mammalian organs and excreta.METHODSTo examine the effect of dietary protein content on turnover (delay and turnover rate), we fed 18 rats either a diet at protein maintenance or above protein maintenance, and quantified their isotopic carbon and nitrogen turnover in ten organs and excreta. These included the excreta faeces and urine, the visceral organs blood plasma, liver, kidney, lung and spleen, the cerebral tissue brain, and the muscular tissues heart and muscle. For data analysis, we used piecewise linear/non‐linear exponential modelling that allows quantifying delay and turnover rate simultaneously.RESULTSDelays were ~0.5 days for carbon and nitrogen turnover and were not affected by dietary protein content. Half‐lives during the following reaction progress were in the range of 1 to 45 days, increasing from excreta to visceral organs to muscular and cerebral organs. Rats fed the higher protein amount had 30% shorter nitrogen half‐lives, and 20% shorter carbon half‐lives.CONCLUSIONSThe renewal times of organs and excreta depend on the dietary protein content. Hence, isotopic diet reconstruction is confronted with variation in half‐lives within the same organ or excrement, altering the time window through which information can be perceived. Copyright © 2013 John Wiley & Sons, Ltd.
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DOI：
10.1002/rcm.3693
发表时间：
2008-09
期刊：
Rapid communications in mass spectrometry : RCM
影响因子：
--
作者：
A. Zazzo;A. Moloney;F. Monahan;C. Scrimgeour;O. Schmidt
通讯作者：
A. Zazzo;A. Moloney;F. Monahan;C. Scrimgeour;O. Schmidt
影响因子：
2
作者：
Trueman, C. N.;MacKenzie, K. M.;Palmer, M. R.
通讯作者：
Palmer, M. R.
影响因子：
1.6
作者：
Bauchinger, Ulf;McWilliams, Scott
通讯作者：
McWilliams, Scott
影响因子：
7.1
作者：
PANNEMANS, DLE;HALLIDAY, D;KESTER, ADM
通讯作者：
KESTER, ADM
影响因子：
5.2
作者：
Kurle, Carolyn M.
通讯作者：
Kurle, Carolyn M.