PCR detection and identification of oral streptococci in saliva samples using gtf genes
PCR detection and identification of oral streptococci in saliva samples using gtf genes
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DOI：
10.1016/j.diagmicrobio.2003.10.002
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发表时间：
2004-03-01
影响因子：
2.9
通讯作者：
Fujiwara, T
中科院分区：
文献类型：
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作者：
Hoshino, T;Kawaguchi, M;Fujiwara, T
口腔链球菌是牙菌斑的主要成分，其流行与各种病理有关。因此，准确鉴定口腔链球菌对于致龋斑块的研究和感染性心内膜炎的诊断具有重要意义。许多口腔链球菌具有合成葡聚糖的葡糖基转移酶，葡聚糖是牙菌斑的必需成分。我们建立了一种快速、精确的方法，利用葡萄糖基转移酶基因的物种特异性区域，通过 PCR 来鉴定口腔链球菌。利用物种特异性引物，可以成功区分变形链球菌、S. sobrinus、S. salivarius、S. sanguinis、S.oralis 和 S. gordonii。此外，我们开发了一种从唾液中提取细菌 DNA 的简单方法。使用所得 DNA 作为模板，进行了所提出的 PCR 检测。它们的分布与常规生化测试的结果一致。这些发现表明，本发明的PCR方法可用于口腔链球菌的分析，并且可以成功地用于临床应用以鉴定与口腔传染病和/或心内膜炎相关的病原菌。 (C) 2004 Elsevier Inc. 保留所有权利。
Oral streptococci are major constituents of dental plaque, and their prevalence is implicated in various pathologies. Therefore, accurate identification of oral streptococci would be valuable for studies of cariogenic plaque and for diagnostic use in infective endocarditis. Many oral streptococci possess glucosyltransferase enzymes that synthesize glucan, which is an obligate component of dental plaque. We established a rapid and precise method to identify oral streptococci by PCR using the species-specific region from the glucosyltransferase gene. With the species-specific primers, Streptococcus mutans, S. sobrinus, S. salivarius, S. sanguinis, S. oralis, and S. gordonii could be successfully distinguished. Further, we developed a simple method to extract the bacterial DNA from saliva. Using the resultant DNA as a template, the proposed PCR detection was performed. Their distribution was in accord with results of conventional biochemical tests. These findings indicate that the present PCR method is useful for the analysis of oral streptococci and can be successfully used in clinical applications to identify pathogenic bacteria associated with oral infectious disease and/or endocarditis. (C) 2004 Elsevier Inc. All rights reserved.