Functional genomic analysis of potato tuber life-cycle
Functional genomic analysis of potato tuber life-cycle
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DOI：
10.1007/bf02360536
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发表时间：
2000-01-01
期刊：
影响因子：
2.9
通讯作者：
Visser, R
中科院分区：
文献类型：
--
作者：
Bachem, C;Van Der Hoeven, R;Visser, R
马铃薯块茎生命周期由许多个人发育阶段组成，包括块茎形成，块茎发育，休眠和发芽。我们已经使用cDNA-AFLP指纹识别来分析从被盗的形成到发芽的期间，在24个单个发育阶段中的基因表达。除了这些发育阶段外，还分析了不同的组织以评估组织特异性，并纳入了各种对照以确定过程特异性。总共将大约18000个转录本衍生的cDNA片段（TDF）可视化，从中，大约2600个统计分析中包括了有关在发育过程中基因表达的一般结论。在整个马铃薯块茎生命周期中分离并测序了200多个工艺特定的TDF。这些TDF与已知基因的序列相似性可深入了解在结构，休眠和发芽过程中发生的过程的种类。
Potato tuber life-cycle is composed of many individual developmental stages including tuber formation, tuber development, dormancy and sprouting. We have used cDNA-AFLP fingerprinting to analyse gene expression in 24 individual stages of development, over the period from stolen formation through sprouting. In addition to these developmental stages, different tissues were analysed to assess tissue specificity and various controls were incorporated to determine process specificity. In total around 18000 transcript derived cDNA fragments (TDFs) were visualised from which circa 2600 were included in a statistical analysis allowing general conclusions about gene expression during development. More than 200 process specific TDFs were isolated and sequenced throughout the potato tuber life-cycle. The sequence similarities of these TDFs to known genes give an insight into the kinds of processes occurring during tuberisation, dormancy and sprouting.