Effects of herbivory by the leaf beetle Ophraella communa on volatile compounds of the invasive common ragweed Ambrosia artemisiifolia.
Effects of herbivory by the leaf beetle Ophraella communa on volatile compounds of the invasive common ragweed Ambrosia artemisiifolia.
复制标题
DOI：
10.3969/j.issn.1674-0858.2021.04.23
复制
发表时间：
2021-01-01
期刊：
影响因子：
--
通讯作者：
Zhou Zhong-Shi
中科院分区：
文献类型：
--
作者：
Cui Shao-Wei;Ma Chao;Zhou Zhong-Shi
植物在遭受植食性昆虫侵害后，会系统地释放植食性诱导的植物挥发物（HIPVs）。为了解广聚萤叶甲取食对入侵豚草豚草挥发性成分的影响，采用动态顶空吸附法和气相色谱-质谱联用技术（GC-MS）分析了广聚萤叶甲取食和机械取食对豚草挥发性成分及相对含量的影响。结果表明，健康植株与受害植株的挥发性成分及其相对含量存在显著差异。与健康植物相比，机械损伤后的植物立即释放出乙酸芳樟酯、桉树脑、十二醛、植醇、-[gamma]-Muurolen、植醇和[beta]-Bourbonene，表明这些化合物是组成型挥发性有机化合物（VOCs）。取食损伤后，大根香叶烯D的相对含量增加，表明其对VOCs有诱导作用。与健康植株和机械损伤植株相比，取食损伤植株释放出特有的化学物质包括桧烯、辛醛、[beta]-罗勒烯和香茅醇，表明它们是由取食诱导产生的，这些挥发物是HIV-1诱导产物。此外，机械损伤和取食损伤对豚草的影响有差异，前者造成的挥发物迅速减少后释放的损害回到健康植物的水平在3天，而后者影响挥发物逐渐减少。此外，机械损伤和取食损伤的挥发物释放时间模式不同，绿色叶的挥发物释放在机械损伤后的早期，而取食损伤后的晚期。本研究结果显示，豚草植物在受到专门的食草动物O. communa。
Plants can systematically release herbivore-induced plant volatiles (HIPVs) after being damaged by herbivorous insects. To understand the effects of herbivory by the leaf beetle Ophraella communa on volatile compounds of the invasive common ragweed Ambrosia artemisiifolia, the changes of volatile compounds and relative contents of common ragweed before and after mechanical and feeding damages by the beetle were collected and analyzed by dynamic headspace adsorption and gas chromatography-mass spectrometry (GC-MS). The results showed that there were significant differences in the volatile compounds and relative contents between healthy and damaged plants. Compared with healthy plants, mechanically damaged plants instantly released Linalyl acetate, Eucalypto, Dodecanal, Phytol, -[gamma]-Muurolen, phytol and [beta]-Bourbonene, suggesting that these compounds were constitutive volatile organic compounds (VOCs). After being damaged by feeding, the plant increased relative contents of Germacrene D, suggesting its inducing VOCs. Compared with healthy plants and mechanically damaged plants, the feeding damaged plant released unique chemical including sabinene, octanal, [beta]-Ocimene and Citronellol, suggesting that they were induced by the feeding treatment, these volatiles were HIVPs. Moreover, the effects of mechanical damage and feeding damage had differential effects on ragweed, the former causing a rapid decrease in volatiles released post damage back to the level of healthy plants at 3 days while the latter effecting a gradual decrease in volatiles. In addition, temporal pattern of volatile release differed between mechanical and feeding damage, with the volatiles of green leaves being released at an early stage after mechanical damage while at a late stage after feeding damage. The results from this study suggested that the common ragweed plants could be induced to release volatiles in response to feeding damage by specialist herbivore O. communa.